Home Shop MMP-9 (catalytic domain) (human), (recombinant, E. coli)

MMP-9 (catalytic domain) (human), (recombinant, E. coli)

BML-SE360

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Naturally-occurring active form of MMP-9
High purity
High activity

Matrix metallopeptidase 9 (MMP-9) belongs to a class of enzymes that belong to the zinc-metalloproteinase family involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, angiogenesis, bone development, wound healing, cell migration, learning and memory. Its also associated with numerous pathological processes, including cancer, immunologic and cardiovascular diseases.

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MMP-9 (catalytic domain) (human), (recombinant, E. coli) SDS-PAGE — SDS-PAGE Analysis: Lane 1: MW Marker, Lane 2: 1μg MMP-9 (catalytic domain) (human), (recombinant, E. coli).

Product Details

Activity	Yes
Alternative Name	Matrix metalloproteinase 9, Gelatinase B, 92 kDa Type IV collagenase
Application Notes	Useful tool to study enzyme kinetics, cleave target substrates, and screen for inhibitors.
Formulation	Liquid. In 50mM TRIS, pH 7.5, containing 1mM calcium chloride, 300mM sodium chloride, 5μM zinc chloride, 0.1% Brij-35 and 15% glycerol.
MW	39 kDa
Purity	≥95% (SDS-PAGE)
Purity Detail	Purified by multi-step chromatography.
Source	Produced in E. coli. Active recombinant matrix metalloproteinase-9 (MMP-9, gelatinase B, 92 kDa type IV collagenase) cloned from human cDNA. The enzyme consists of residues Phe¹⁰⁷-Pro⁴⁴⁹ (NM_004994), which comprises the catalytic/fibronectin domain of human MMP-9, with a C-terminal purification tag. This represents a naturally-occurring active form of MMP-9 which lacks the C-terminal hemopexin domain. Activity toward its targets, such as gelatin, casein, or peptide substrates, is unaffected.
Specific Activity	≥500 pmol/min/ug at 37°C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100 µM; Prod. No. BML-P125) as substrate.
UniProt ID	P14780

Handling & Storage

Handling	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Toll-like receptor 2, hyaluronan, and neutrophils play a key role in plaque erosion: the OPTICO-ACS study: D. Meteva, et al.; Eur. Heart J. 44, 3892 (2024), Abstract
Mechanical strain modulates extracellular matrix degradation and byproducts in an isoform-specific manner: A. Yeganegi, et al.; Biochim. Biophys. Acta Gen. Subj. 1867, 130286 (2023), Abstract
An Activity-Based Nanosensor for Minimally-Invasive Measurement of Protease Activity in Traumatic Brain Injury: Kudryashev, J. A., Madias, M. I., et al.; Adv. Funct. Mater. 33, (2023), Abstract
Validation of Matrix Metalloproteinase-9 (MMP-9) as a Novel Target for Treatment of Diabetic Foot Ulcers in Humans and Discovery of a Potent and Selective Small-Molecule MMP-9 Inhibitor That Accelerates Healing: T.T. Nguyen, et al.; J. Med. Chem. 61, 8825 (2021), Abstract
Cell-specific expression of the transcriptional regulator RHAMM provides a timing mechanism that controls appropriate wound re-epithelialization: C. Tolg, et al.; J. Biol. Chem. 295, 5427 (2020), Abstract — Full Text
Substitutions for arginine at position 780 in triple helical domain of the α1(I) chain alter folding of the type I procollagen molecule and cause osteogenesis imperfecta: E. Makareeva, et al.; PLoS One 13, e0200264 (2018), Abstract — Full Text
Nucleic acid-induced potentiation of matrix metalloproteinase-9 enzymatic activity: T. Duellman, et al.; Biochem. J. 475, 1597 (2018), Abstract — Full Text
Toxicological effects of NCKU-21, a phenanthrene derivative, on cell growth and migration of A549 and CL1-5 human lung adenocarcinoma cells: H.F. Liao, et al.; PLoS One 12, e28945763 (2017), Abstract — Full Text
dCas9-mediated transcriptional activation of tissue inhibitor of metalloproteinases: T. Duellman, et al.; Metalloproteinases Med. 4, 63 (2017), Abstract — Full Text
Active Matrix Metalloprotease-9 Is Associated with the Collagen Capsule Surrounding the Madurella mycetomatis Grain in Mycetoma: K. Geneugelijk, et al.; PLoS Negl. Trop. Dis. 8, e2754 (2014), Application(s): Gelatin Zymography, Abstract — Full Text