MMP-8 proenzyme (human neutrophils)

Matrix metalloproteinase 8, Neutrophil collagenase, Collagenase-2

100µg/ml

Liquid. In 50mM TRIS-HCl, pH 7.0, containing 200mM NaCl, 5mM CaCl₂, 1µM ZnCl₂, 0.05% BRIJ 35, and 0.05% sodium azide.

~85kDa.

≥90% (SDS-PAGE)

Isolated from stimulated human neutrophil granulocytes (buffy coat). Requires activation.

≥60mU/mg protein (Y. Masui, et al.; Biochem. Med. 17, 215 (1977)). One unit is defined as the amount of enzyme that hydrolyzes 1µmol 2,4-dinitrophenyl-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0.

Precursor enzyme needs activation using 2mM AMPA (aminophenylmercuric acetate) or 1mM mersalylic acid for 60 min. at 37°C. Alternatively use 0.1mM PCMB (p-chloromercuribenzoate) or 10µg/ml trypsin for 20 min. at 25°C; PCMB is substantially more effective.

P22894