MMP-7 (catalytic domain) (human), (recombinant)

Preincubation of MMP-7 catalytic domain at 19nM with the broad-spectrum inhibitor GM6001 (Prod. No. BML-EI300) at 100nM for 1 hour completely inhibits enzymatic activity.

Matrix metalloproteinase 7, Matrilysin, Pump

Useful tool to study enzyme kinetics, cleave target substrates, and screen for inhibitors.

Liquid. In 50 mM Tris-HCl pH 7.5, 5 mM CaCl₂, 300 mM NaCl, 20 µM ZnCl₂, 0.5% Brij-35, and 30% glycerol.

20.4 kDa

≥95% (SDS-PAGE)

Purified by multi-step chromatography.

Produced in E. coli. Active Matrix Metalloproteinase-7 (MMP-7, matrilysin, pump) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-7 (Tyr⁹⁵-Lys²⁶⁷, NM_002423) with a C-terminal purification tag.

≥200 pmol/min/µg at 37°C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-GlyOEt (100 µM; Prod. No. BML-P125) as substrate.

P09237