MMP-19 (catalytic domain) (human), (recombinant)

Preincubation of MMP-19 catalytic domain at 1.9µM with the broad-spectrum inhibitor GM6001 (Prod No. BML-EI300) at 100nM for 30 minutes only weakly inhibits enzymatic activity (27%).

Matrix metalloproteinase 19, RASI

Useful tool to study enzyme kinetics, cleave target substrates, and screen for inhibitors.

Liquid. In 25mM NaBorate, pH 9.3, containing 5mM CaCl₂, 20μM ZnCl₂, 0.05% Brij-35 and 20% glycerol.

19.2 kDa

≥95% (SDS-PAGE)

Purified by multi-step chromatography.

Recombinant MMP-19 corresponding to aa Leu⁹⁹-Ser²⁵⁹ (NM_002429), which comprises the catalytic domain of human MMP-19, with a C-terminal purification tag.

Produced in E.coli. Active recombinant MMP-19.

≥ 0.40U/µg. One U=100 pmol/min at 37°C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100µM; Prod. No. BML-P125) as substrate.

Q99542