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This sequence, KKRAARATSDVFA, is chicken gizzard smooth muscle myosin P-light chain (MLC 11-23) with Pro to Ala14 and Gln to Ala15 substitutions. Contains specificity determinants for MLC phosphorylation (target is Ser19) and is an excellent myosin light chain kinase (MLCK) substrate. Km=8.6 µM for rabbit skeletal muscle MLCK, 7.5 µM for chicken gizzard smooth muscle MLCK.
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RUO – Research Use Only |
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