MitoPT® TMRM Assay

The MitoPT TMRM Assay detects mitochondrial membrane depolarization utilizing the fluorescent dye TMRM. When accumulated in a negatively charge polarized mitochondria, TMRM fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, TMRM reagent is dispersed through the cell cytosol and fluorescence levels drop dramatically. Analyze your results using a flow cytometer, fluorescence plate reader, or fluorescence microscopy.