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Mitochondria/Cytosol fractionation kit

ALX-850-276

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The core kit contains unique formulations of buffers and reagents that allow isolation of mitochondria and cytosol in only two easy steps. No ultracentrifugations are required. No toxic chemicals are involved.

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Product Details

Application Notes	For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.
Contents	10ml Mitochondria Extraction Buffer, 20ml 5X Cytosol Extraction Buffer, 100µl DTT (1M), 1 vial Protease Inhibitor Cocktail.
Quantity	~100 tests.
Species Reactivity	Mammalian

Handling & Storage

Handling	Protect from light. Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Aflatoxin B1 exposure triggers hepatic lipotoxicity via p53 and perilipin 2 interaction-mediated mitochondria-lipid droplet contacts: An in vitro and in vivo assessment: L. Che, et al.; J. Hazard. Mater. 445, 130584 (2023), Abstract
Protein phosphatase 2A-B56γ-Drp1-Rab7 signaling axis regulates mitochondria-lysosome crosstalk to sensitize the anti-cancer therapy of hepatocellular carcinoma: L. Che, et al.; Biochem. Pharmacol. 202, 115132 (2022), Abstract
Bone morphogenetic protein inhibitors and mitochondria targeting agents synergistically induce apoptosis-inducing factor (AIF) caspase-independent cell death in lung cancer cells: A. Mondal, et al.; Cell Commun. Signal. 20, 99 (2022), Abstract
Ym155 localizes to the mitochondria leading to mitochondria dysfunction and activation of AMPK that inhibits BMP signaling in lung cancer cells: A. Mondal, et al.; Sci. Rep. 12, 13135 (2022), Abstract
Targeting bone morphogenetic protein receptor 2 sensitizes lung cancer cells to TRAIL by increasing cytosolic Smac/DIABLO and the downregulation of X-linked inhibitor of apoptosis protein: R. NeMoyer, et al.; Cell Commun. Signal. 17, 150 (2019), Abstract — Full Text
Nifurtimox reduces N-Myc expression and aerobic glycolysis in neuroblastoma: K.M. Cabanillas Stanchi, et al.; Cancer Biol. Ther. 16, 1353 (2015), Application(s): Flow cytometry using neuroblastoma cells, Abstract — Full Text
MAPK/ERK Activation Sensitizes MKN-28 Cells to Cisplatin-Induced Apoptosis: Z. G. Zhang, et al.; Cancer Stud. Mol. Med. Open J. 2, 52 (2015), Application(s): Western Blot Assay, Full Text
Green tea epigallocatechin 3-gallate accumulates in mitochondria and displays a selective antiapoptotic effect against inducers of mitochondrial oxidative stress in neurons: E.K. Schroeder, et al.; Antioxid. Redox Signal. 11, 469 (2009), Abstract