MITO-ID^® Red detection kit (GFP-CERTIFIED^®)

• Mitochondria-selective dye stains live, detergent permeabilized and aldehyde fixed cells
• Long wavelength red emission is easily multiplexed with common fluorescent dyes
• Highly resistant to photobleaching and concentration quenching, for strong, consistent fluorescent signal
• Highlights mitochondria regardless of the organelle’s membrane potential status
• Stringently manufactured, to control and eliminate non-specific assay artifacts

MITO-ID^® Red Detection Kit (GFP-CERTIFIED^®) contains a proprietary membrane-permeable mitochondria-selective dye suitable for use with live-, detergent-permeabilized- and even aldehyde-fixed-cells. Unlike conventional dyes, such as DiOC6(3), JC-1, rhodamine 123 and tetramethylrhodamine ethyl ester, MITO-ID^® Red dye highlights mitochondria regardless of their energetic state. The dye is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as, High Content Screening (HCS) platforms. The kit is useful for assessing mitochondrial morphology changes, estimating mitochondrial mass and co-localizing GFP-tagged proteins to the mitochondrial compartment. This kit is specifically designed for use with GFP expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with fluorescent probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties such as fluorescein and coumarin. A nuclear counterstain (Hoechst 33342) is provided to highlight this organelle as well. Wavelength maxima: MITO-ID^® Red λex 558 nm, λem 690 nm; Hoechst 33342 λex 350 nm, λem 461 nm.