Microcystin-LR monoclonal antibody (MC10E7)

With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody MC10E7, IC₅₀ values for microcystin-LR of 0.06µg/l have been obtained. The provisional guideline value proposed by the WHO is 1 µg/l for drinking water. The detection limit for microcystin-LR is 0.006 µg/l. All microcystin variants containing an arginine at position 4 show similar IC₅₀ values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for MC10E7 was determined to be at least 7×10¹⁰ l/mol.The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1µg/l were measured and a mean recovery of 99.9±16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.

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Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 aa 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-aa.