Home Shop MHC Class I monoclonal antibody (W6/32) (R-PE conjugate)

MHC Class I monoclonal antibody (W6/32) (R-PE conjugate)

ALX-805-711R

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HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

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This antibody is covered by our Worry-Free Guarantee.

Product Details

Alternative Name	HLA Class I
Application	Flow Cytometry
Clone	W6/32
Crossreactivity	Does not cross-react with rabbit MHC class I.
Formulation	Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
Host	Mouse
Immunogen	Membrane of human tonsil cells.
Isotype	IgG2a
Purity Detail	Protein G-affinity purified. The purified antibody is conjugated with R-Phycoerythrin (PE). The conjugate is purified by size-exclusion chromatography.
Recommendation Dilutions/Conditions	Flow Cytometry (1:50)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Bovine, Cat, Human, Monkey
Specificity	Recognizes virtually all nucleated human cells. Suitable as positive control for HLA tissue typing and crossmatching.
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Do not freeze. Protect from light.
Short Term Storage	+4°C
Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Expression of HLA-G in human cornea, an immune-privileged tissue: M. Le Discorde, et al.; Hum. Immunol. 64, 1039 (2003), Abstract
HLA-B27 misfolding is associated with aberrant intermolecular disulfide bond formation (dimerization) in the endoplasmic reticulum: N.S. Dangoria, et al.; J. Biol. Chem. 277, 23459 (2002), Abstract — Full Text
The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex: T.M. Tran, et al.; Immunogenetics 53, 440 (2001), Abstract
Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody: J.J. Ladasky, et al.; Immunogenetics 49, 312 (1999), Abstract
Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin: M.J. Shields and R.K. Ribaudo; Tissue Antigens 51, 567 (1998), Abstract
Major histocompatibility complex class I molecule expression is normal on peripheral blood lymphocytes from patients with insulin-dependent diabetes mellitus: W. Hao, et al.; J. Clin. Invest. 98, 1613 (1996), Abstract — Full Text
Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species: C.N. Jacobsen, et al.; Vet. Immunol. Immunopathol. 39, 461 (1993), Abstract
Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin: F. Kievits and P. Ivanyi; Hum. Immunol. 50, 115 (1987), Abstract
Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast: P.L. Stern, et al.; J. Immunol. 138, 1088 (1987), Abstract
Quantitative analysis of platelet surface HLA by W6/32 anti-HLA monoclonal antibody: K.J. Kao, et al.; Blood 68, 627 (1986), Abstract — Full Text

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