Home Shop MHC Class I monoclonal antibody (W6/32)

MHC Class I monoclonal antibody (W6/32)

ALX-805-711

Datasheet
Manual
SDS
CofA

Already have an account?

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Shipping: Available products typically ship within 24/48h, via priority shipping.

Do you need support? Contact Customer Service or Technical Support.

Online Account
Access or Create Your Account

This antibody is covered by our Worry-Free Guarantee.

Product Details

Alternative Name	HLA Class I
Application	ELISA, Flow Cytometry, FUNC, ICC, IHC (FS), IP, WB
Clone	W6/32
Crossreactivity	Does not cross-react with rabbit MHC class I.
Formulation	Liquid. In PBS, pH 7.4. 15mM sodium azide.
Host	Mouse
Immunogen	Membrane of human tonsil cells.
Isotype	IgG2a
Purity Detail	Purified by protein-A affinity chromatography.
Recommendation Dilutions/Conditions	Western Blot (non-reducing)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Bovine, Cat, Human, Monkey
Specificity	Recognizes virtually all nucleated human cells. Suitable as positive control for HLA tissue typing and crossmatching.
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Short Term Storage	+4°C
Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Characterization of the functional and transcriptomic effects of pro-inflammatory cytokines on human EndoC-βH5 beta cells.: Frørup, C., Gerwig, R., et al.; Front. Endocrinol. (Lausanne) 14, 1128523 (2023), Application(s): ICC-IF, WB / Reactant(s): Human, Abstract
Characterisation of the functional and transcriptomic effects of pro-inflammatory cytokines on human EndoC-βH5 beta cells: Frørup, C., Gerwig, R., et al.; bioRxiv , (2022)
Human cytomegalovirus evades antibody-mediated immunity through endoplasmic reticulum-associated degradation of the FcRn receptor.: Liu, X., Palaniyandi, S., et al.; Nat. Commun. 10, 3020 (2019), Application(s): WB, Abstract
Expression of HLA-G in human cornea, an immune-privileged tissue: M. Le Discorde, et al.; Hum. Immunol. 64, 1039 (2003), Abstract
HLA-B27 misfolding is associated with aberrant intermolecular disulfide bond formation (dimerization) in the endoplasmic reticulum: N.S. Dangoria, et al.; J. Biol. Chem. 277, 23459 (2002), Abstract — Full Text
The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex: T.M. Tran, et al.; Immunogenetics 53, 440 (2001), Abstract
Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody: J.J. Ladasky, et al.; Immunogenetics 49, 312 (1999), Abstract
Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin: M.J. Shields and R.K. Ribaudo; Tissue Antigens 51, 567 (1998), Abstract
Major histocompatibility complex class I molecule expression is normal on peripheral blood lymphocytes from patients with insulin-dependent diabetes mellitus: W. Hao, et al.; J. Clin. Invest. 98, 1613 (1996), Abstract — Full Text
Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species: C.N. Jacobsen, et al.; Vet. Immunol. Immunopathol. 39, 461 (1993), Abstract