Together with FeSO4 MGD is a useful component for the formation of the MGD2-Fe2+complex, which is an excellent nitric oxide (NO) spin-trapping reagent. The MGD2-Fe2+complex is quite unstable, especially in the presence of dissolved oxygen. Thus, the complex should be used immediately after being made. An excess (usually 5-fold excess), of MGD to Fe2+ is used for making the complex with FeSO4 to give a more stable complex solution. Acidic conditions should be avoided because dithiocarbamate tends to decompose forming toxic carbon disulfide. It was reported that MGD and Fe(MGD)2 do not exhibit toxicity up to 8mmol/kg and 0.3mmol/kg, respectively.
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