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Immunofluorescence staining of MFN2 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MFN2 polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunochemical staining of human MFN2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
MFN2 was immunoprecipitated using: Lane A:0.5 mg HeLa Jurkat Whole Cell Lysate, 4 µL anti-MFN2 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MFN2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique.Performed under reducing conditions. Observed band size:86 kDa.
Anti-MFN2 rabbit polyclonal antibody at 1:500. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:86 kDa.
| Regulatory Status |
RUO – Research Use Only |
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