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- Highly active MEGACD40L® oligomer mimics in vivo membrane-assisted CD40L aggregation and stimulation without the need for enhancers
- High purity with low endotoxin levels
- Experienced manufacturing results in consistent and dependable lot-to-lot performance
- Optimized formulation for improved stability and enhanced activity
MEGACD40L® is a high activity protein in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30/adiponectin. This protein very effectively simulates the natural membrane-assisted aggregation of CD40L. It provides a simple and equally potent alternative to [CD40L+enhancer] combinations (Prod. No. ALX-850-075).
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B cell lymphocytes activation after titration with MEGACD40L® (soluble) (mouse) (rec.). Method: PBMCs (Peripheral Blood Mononuclear Cells) were incubated at 37°C, 5% CO2 for 48 hours in 48 well plates (1 x 106 cells per well) containing 200µl serum free test media with or without 1000ng/mL MEGACD40L® (soluble) (mouse) (rec.) (Prod. No. ALX-522-120). After treatment the cells were washed 3X with PBS + 1% BSA. The cells were dual stained for 2 hours at 4°C with mouse anti-human CD19 (PE conjugate) and mouse anti-human CD86 (APC conjugate). The cells were washed 2X with PBS + 1% BSA, then re-suspended In PBS. Samples were analyzed by on a BD Facs Calibur flow cytometer. The data is presented as the percent of CD86 positive cells per CD19 positive B cells with or without treatment with 1000ng/mL mega mouse CD40L.
Figure 1: Graphical representation of MEGACD40L® (soluble) (mouse) (recombinant) (Prod. No. ALX-522-120).
Coomassie stained SDS-PAGE. Lane 1: Molecular weight marker. Lane 2: 1.0 µg MEGACD40L® (soluble) (mouse) (rec.) (Prod. No. ALX-522-120).
| Regulatory Status |
RUO – Research Use Only |
|---|
Related Products
MEGACD40L® Protein (soluble) (human), (recombinant)
ALX-522-110
High activity, high purity CD40L protein for co-stimulatory activation of an immune response
| Alternative Name | CD40L:ACRP30headless, CD154:ACRP30headless, TNFSF 5:ACRP30headless, gp39:ACRP30headless |
|---|---|
| Purity | ≥90% (SDS-PAGE) |
| Source | Produced in CHO cells. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag. |
| Alternative Name | Tail-Adp, Tail Adiponectin, Adipocyte complement related protein of 30kDa headless |
|---|---|
| Purity | ≥90% (SDS-PAGE) |
| Source | Produced in HEK 293 cells. The collagen domain of mouse ACRP30 (aa 18-111) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag. |