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ACE2 substrate
Fluorogenic substrate for angiotensin converting enzyme-2 (ACE2), a zinc-dependent carboxypeptidase with 42% amino acid sequence identity to ACE1. Unlike Mca-YVADAPK(Dnp), this substrate is not cleaved by caspases. Mca fluorescence is quenched by the Dnp group until cleavage (at Pro-Lys) separates them. Ex: 328 nm, Em: 393 nm, although the following Ex/Em have also been used: 320-340/400-420. This highly quenched (99.6%) substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic Mca control peptide (BML-P127).
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Product Details
| Alternative Name |
ACE2 substrate (fluorogenic) |
|---|---|
| Appearance |
Yellow powder. |
| Formula |
C32H37N6O12 |
| Identity |
Determined by MS. |
| MW |
696.7 |
| Purity |
≥95% (HPLC) |
| Sequence |
Mca-Ala-Pro-Lys(Dnp) [Mca=(7-methoxycoumarin-4-yl)acetyl;Dnp=2,4-dinitrophenyl] |
| Solubility |
Soluble in DMSO or 10% Ammonium Hydroxide (2 mg/ml). |
| Source |
Synthetic. |
Handling & Storage
| Use/Stability |
As indicated on product label or CoA when stored as recommended. Stock solutions in DMSO stable at -20°C |
|---|---|
| Handling |
Protect from light and moisture. |
| Long Term Storage |
-20°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Differential Effects of Losartan and Finerenone on Diabetic Remodeling, Oxidative Stress and ACE Activity in the Gastrointestinal Tract of Streptozotocin-Induced Diabetic Rats: Esteves-Monteiro, M., Vitorino-Oliveira, C., et al.; Int. J. Mol. Sci. 26, 6294 (2025), Abstract
- SGLT2i and GLP1-RA exert additive cardiorenal protection with a RAS blocker in uninephrectomized db/db mice: Martos-Guillami, N., Vergara, A., et al.; Front. Pharmacol. 15, 1415879 (2024), Reactant(s): Mouse, Abstract
- Synthesis of cannabidiol-based compounds as ACE2 inhibitors with potential application in the treatment of COVID-19: G. V. L. Marques, et al.; Eur. J. Med. Chem. 260, 115760 (2023), Abstract
- Plasmatic renin-angiotensin system in normotensive and hypertensive patients hospitalized with COVID-19: M.G. Silva, et al.; Biomed. Pharmacother. 152, 113201 (2022), Abstract
- Enhanced Cardiorenal Protective Effects of Combining SGLT2 Inhibition, Endothelin Receptor Antagonism and RAS Blockade in Type 2 Diabetic Mice: Vergara, A., Jacobs-Cachá, C., et al.; Int. J. Mol. Sci. 23, (2022), Reactant(s): Mouse, Abstract
- Exploring Renal Changes after Bariatric Surgery in Patients with Severe Obesity: A. Oliveras, et al.; J. Clin. Med. 11, 728 (2022), Abstract
- Dysregulation of ACE-1 in Normal Aging and the Early Stages of Alzheimer’s Disease: R. MacLachlan, et al.; J. Gerontol. A Biol. Sci. Med. Sci. 77, 1775 (2022), Abstract
- Activation of angiotensin-converting enzyme 2/angiotensin (1–7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling: R. Dang, et al.; Aging Cell 20, 13480 (2021), Abstract
- Apelin-13 in septic shock: effective in supporting hemodynamics in sheep but compromised by enzymatic breakdown in patients: D. Coquerel, et al.; Sci. Rep. 11, 22770 (2021), Abstract
- SARS-CoV-2 Spike Protein Regulation of Angiotensin Converting Enzyme 2 and Tissue Renin-Angiotensin Systems: Influence of Biologic Sex: Ensor, C. M., Alsiraj, Y., et al.; bioRxiv , (2021)
- Both Specific Endothelial and Proximal Tubular Adam17 Deletion Protect against Diabetic Nephropathy: V. Palau, et al.; Int. J. Mol. Sci. 22, 5520 (2021), Abstract
- Metabolism of angiotensin peptides by angiotensin converting enzyme 2 (ACE2) and analysis of the effect of excess zinc on ACE2 enzymatic activity: Y. Polak & R.C. Speth; Peptides 137, 170477 (2021), Abstract
- Male bias in ACE2 basic science research: Missed opportunity for discovery in the time of COVID-19: B. Stanic., et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 1152, 356 (2021), Abstract
- A pilot study to assess the circulating renin-angiotensin system in COVID-19 acute respiratory failure: D.C. Files, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 321, L213 (2021), Abstract
- SARS-CoV-2 Infection Modulates ACE2 Function and Subsequent Inflammatory Responses in Swabs and Plasma of COVID-19 Patients: L. Gutiérrez-Chamorro, et al.; Viruses 13, 1715 (2021), Abstract
- Role of the Renin Angiotensin System in Blood Pressure Allostasis-induced by Severe Food Restriction in Female Fischer rats: A.M.A. de Souza, et al.; Sci. Rep. 8, 10327 (2018), Abstract
- Chymase-dependent production of angiotensin II: an old enzyme in old hearts: G. Froogh, et al.; Am. J. Physiol. Heart Circ. Physiol. 312, H223 (2017), Abstract
- Serum activity of angiotensin converting enzyme 2 is decreased in patients with acute ischemic stroke: D.M. Bennion, et al.; J. Renin Angiotensin Aldosterone Syst. 17, 1470320316661060 (2016), Reactant(s): Human, Abstract — Full Text
- Estradiol, acting through ERα, induces endothelial non-classic renin-angiotensin system increasing angiotensin 1-7 production: A. Mompeon, et al.; Mol. Cell. Endocrinol. 422, 1 (2016), Abstract
- Urinary Angiotensin Converting Enzyme 2 Increases in Patients With Type 2 Diabetic Mellitus: Y. Liang, et al.; Kidney Blood Press. Res. 40, 101 (2015), Application(s): Fluorescent Substrate-Based Assay, Abstract — Full Text
- Administration of 17β-estradiol to ovariectomized obese female mice reverses obesity-hypertension through an ACE2-dependent mechanism: Wang, Y., Shoemaker, R., et al.; Am. J. Physiol. Endocrinol. Metab. 308, E1066 (2015), Reactant(s): Mouse, Abstract
- Angiotensin-converting enzyme-2 (ACE2): comparative modeling of the active site, specificity requirements, and chloride dependence: J.L. Guy, et al.; Biochemistry 42, 13185 (2003), Abstract
- Hydrolysis of biological peptides by human angiotensin-converting enzyme-related carboxypeptidase: C. Vickers, et al.; J. Biol. Chem. 277, 14838 (2002), Abstract — Full Text
- ACEH/ACE2 is a novel mammalian metallocarboxypeptidase and a homologue of angiotensin-converting enzyme insensitive to ACE inhibitors: A.J. Turner, et al.; Can. J. Physiol. Pharmacol. 80, 346 (2002), Abstract
- A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9: M. Donoghue, et al.; Circ. Res. 87, E1 (2000), Abstract — Full Text
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