LYSO-ID^® Green detection kit

The LYSO-ID^® Green detection kit contains an acidic organelle-selective dye suitable for live-cell staining. Conventional fluorescent stains for acidic organelles, such as Acridine Orange (Prod. No. ENZ-52405), form metachromatic artifacts that interfere with multicolor imaging applications. LYSO-ID^® Green dye generates emission profiles that can be multiplexed with other fluorophores. The dye accumulates in acidic compartments, such as endosomes, lysosomes, and secretory vesicles. Low micromolar concentrations of LYSO-ID^® Green dye are sufficient for staining mammalian cells. This has been validated with the human cervical carcinoma cell line, HeLa, the human T-lymphocyte cell line, Jurkat, and the human bone osteosarcoma epithelial cell line, U2OS. The LYSO-ID^® Green dye is a new green-emitting, cell-permeable small organic probe molecule that spontaneously localizes to live cell acidic organelles. It can be readily used in combination with other common UV and visible light excitable fluorescent dyes and various fluorescent proteins in multicolor imaging and detection applications. The LYSO-ID^® Green dye is suitable for both short-term and long-term tracking studies. It emits in the FITC region of the visible light spectrum, and is highly resistant to photobleaching, concentration quenching and photoconversion. The LYSO-ID^® Green detection kit is specifically designed for use with RFP-expressing cell lines, as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs). A lysosome perturbation agent, chloroquine, is provided as a positive control for monitoring changes in lysosome number and volume. A nuclear counterstain is also provided in the kit to highlight this organelle as well.