Lyn B (human), (recombinant) (GST-tag)

Tyrosine protein kinase Lyn B

Useful for kinetic and functional studies, phosphorylation of target substrates, drug screening.

Liquid. 0.1 mg/ml in 50 mM TRIS-HCl, pH 7.5, containing 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.

~85 kDa

≥90% (SDS-PAGE)

Produced in insect cells. Full length active enzyme. Produced in a baculovirus expression system.

180 nmol/min/mg. Kinase activity was measured as the molar amount of phosphate incorporated into poly (Glu-Tyr) at 30°C using a concentration of 50μM [³²P]ATP in 25mM MOPS, pH 7.2, 12.5mM β-glycerophosphate, 5mM EGTA, 2mM EDTA, 25mM MgCl₂, 0.25mM DTT (added right before using).

Replacement for ADI-PPK-431

P07948-2