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LY-294002 is a cell permeable highly selective inhibitor of phosphatidylinositol 3-kinase (IC50 = 0.31, 0.73, 1.06 and 6.60 µM for PI 3-Kβ, PI 3-Kα, PI 3-Kδ and PI 3-Kγ respectively). It inhibits autophagy in rat hepatocytes (IC50 = 10 µM). LY-294002 is an extremely useful tool for determining the role of PI 3-K in cellular signaling. It is more stable in solution than Wortmannin, and also blocks autophagosome formation.
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