Home Shop LPS from E. coli, Serotype EH100 (Ra) (TLRGRADE®) (Ready-to-Use)

LPS from E. coli, Serotype EH100 (Ra) (TLRGRADE®) (Ready-to-Use)

ALX-581-010

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TLR4 activator

Potent activator of TLR 4
TLR4-specific activation as shown in TLR4-deficient cells
Convenient and stable ready-to-use solution
Highly cited

TLRGRADE® LPS is commonly used to active TLR4, trigger inflammatory signaling, and co-stimulate the immune response, both in vitro and in vivo.

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Product Details

Alternative Name	Lipopolysaccharide from E. coli, Serotype EH100(Ra)
Appearance	Clear colorless liquid.
Couple Target	TLR, TLR4
Couple Type	Activator, Ligand
Formulation	Liquid. Sterile, ready-to-use solution in double distilled, pyrogen-free water.
Purity	Absence of detectable protein or DNA contaminants with agonistic TLR activity.
Source	Rough (R)-form LPS isolated and purified from E. coli EH100 (Ra-mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended. Stable for at least 1 year after receipt when stored at +4°C.
Handling	Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Use must be restricted to qualified personnel.
Long Term Storage	+4°C
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

Mitochondrial respiratory complex III sustains IL-10 production in activated macrophages and promotes tumor-mediated immune evasion: Zotta, A., Toller-Kawahisa, J. E., et al.; Sci. Adv. 11, eadq7307 (2025), Abstract
Endogenous SLPI contributes to the regulation of inflammatory responses in peritoneal macrophages by modulating MMP-9 production: Tyshchenko, M., Pocałuń, N., et al.; Front. Immunol. 16, 1563845 (2025), Abstract
Metabolic reprogramming of macrophages by PKM2 promotes IL-10 production via adenosine: Toller-Kawahisa, J. E., Viacava, P. R., et al.; Cell Rep. 44, 115172 (2025), Abstract
An integrative epigenome-based strategy for unbiased functional profiling of clinical kinase inhibitors: Gualdrini, F., Rizzieri, S., et al.; Mol. Syst. Biol. 20, 626 (2024), Abstract
Acetyl-CoA production by Mediator-bound 2-ketoacid dehydrogenases boosts de novo histone acetylation and is regulated by nitric oxide: Russo, M., Gualdrini, F., et al.; Mol. Cell 84, 967 (2024), Abstract
Novel Morphological Profiling Assay Connects ex Vivo Endothelial Cell Responses to Disease Severity in Liver Cirrhosis: Postma, R. J., Broekhoven, A. G. C., et al.; Gastro. Hep. Adv. 3, 238 (2024), Abstract
SARM1 regulates pro-inflammatory cytokine expression in human monocytes by NADase-dependent and -independent mechanisms: Sugisawa, R., Shanahan, K. A., et al.; iScience 27, 109940 (2024), Abstract
Dexamethasone impairs the expression of antimicrobial mediators in lipopolysaccharide-activated primary macrophages by inhibiting both expression and function of interferon β: O’Neil, J. D., Bolimowska, O. O., et al.; Front. Immunol. 14, 1190261 (2023), Abstract
Apis mellifera anatoliaca Venom Exerted Anti-Inflammatory Activity on LPS-Stimulated Mammalian Macrophages by Reducing the Production of the Inflammatory Cytokines: S. Sevin, et al.; Appl. Biochem. Biotechnol. 195, 3194 (2023), Abstract
Dimethyl fumarate and 4-octyl itaconate are anticoagulants that suppress Tissue Factor in macrophages via inhibition of Type I Interferon: Ryan, T. A. J., Hooftman, A., et al.; Nat. Commun. 14, 3513 (2023), Abstract

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LPS from E. coli, Serotype EH100 (Ra) (TLRGRADE®) (Ready-to-Use)

ALX-581-010

Product Details

Handling & Storage

Resources

Metabolism

Cellular Therapy

Cardiovascular Research

Drug Discovery

AMPIVIEW® RNA Probes

Immuno-Oncology

AMPIGENE® PCR Solutions

Bioprocess

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

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