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LIMK1 monoclonal antibody (1B11)

ALX-803-343

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The LIM kinases 1 and 2 (LIMK1 and LIMK2) are activated downstream of the Rho-ROCK, Rac-PAK, and Cdc42-MRCK pathways, thereby acting as network nodes in the signaling pathways that regulate the actin cytoskeleton. LIMK is required for matrix remodeling activities for path generation by leading cells in collective invasion. LIMK1 phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton.

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Product Details

Alternative Name	LIM domain kinase 1
Application	ICC, IHC, IP, WB
Clone	1B11
Crossreactivity	Does not cross-react with LIMK2.
Formulation	Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Host	Rat
Immunogen	Recombinant LIM + PDZ domain of LIMK1.
Isotype	IgG2a
Purity Detail	Protein G affinity purified.
Recommendation Dilutions/Conditions	Immunoprecipitation (20ng/ml)Immunohistochemistry (paraffin sections 10µg/ml)Immunocytochemistry (10µg/ml)Western Blot (200ng/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from concentrated hybridoma tissue culture supernatant.
Species Reactivity	Species independent
Specificity	Recognizes full length GFP-LIMK1 and GFP-PDZ domain but not the GFP-LIM or GFP-kinase regions.
UniProt ID	P53667 (human)
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Handling	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Short Term Storage	+4°C
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

LIMK1 variants are associated with divergent endocrinological phenotypes and altered exocytosis dynamics: Muffels, I. J. J., Carter, T., et al.; iScience 28, 112585 (2025), Abstract
LIM-kinase 1 effects on memory abilities and male courtship song in Drosophila depend on the neuronal type.: Zhuravlev, A. V., Zalomaeva, E. S., et al.; Vavilovskii Zhurnal Genet Selektsii 27, 250 (2023), Reactant(s): Drosophila melanogaster, Abstract
LIM kinases: function, regulation and association with human disease: R.W. Scott & M.F. Olson; J. Mol. Med. 85, 555 (2007), Abstract
The phosphorylation of p25/TPPP by LIM kinase 1 inhibits its ability to assemble microtubules: K. Acevedo, et al.; Exp. Cell Res. 313, 4091 (2007), Abstract
Hsp90 increases LIM kinase activity by promoting its homo-dimerization: R. Li, et al.; FASEB J. 20, 1218 (2006), Abstract
LIM kinase 1 coordinates microtubule stability and actin polymerization in human endothelial cells: M. Gorovoy, et al.; J. Biol. Chem. 280, 26533 (2005), Abstract
LIM kinase 1, a key regulator of actin dynamics, is widely expressed in embryonic and adult tissues: V.C. Foletta, et al.; Exp. Cell Res. 294, 392 (2004), Abstract

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LIMK1 monoclonal antibody (1B11)

ALX-803-343

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

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