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LIF (mouse), (recombinant)

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Alternative Name	Leukemia inhibitory factor
Biological Activity	Induces macrophage differentiation and proliferation of hematopoietic stem cells; maintains indifferentiated state of of embryonic stem (ES) cell cultures; inhibits proliferation of the mouse myeloid cell line M1.
Formulation	Liquid. 0.2μm-filtered solution in PBS containing 0.5% BSA. Contains no preservatives. Further dilutions should be made with medium containing 1% BSA.
MW	~20kDa
Purity	≥95% (SDS-PAGE)
Purity Detail	Purified by FPLC.
Quantity	10⁷U
Source	Produced in E. coli.
Technical Info / Product Notes	Maintenance of ES Cells: 1,000U/ml are recommended to maintain indifferentiated ES cells, R1 or E14 cells. M1Growth Inhibition Assay:50units/ml are defined as the concentration of LIF that induces 50% of differentiated cells in tissue culture medium.
UniProt ID	P09056

Use/Stability	Stable for 6 months when stored at +4°C.
Handling	Avoid freeze/thaw cycles.
Short Term Storage	+4°C
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Osteogenic Effect of Inducible Nitric Oxide Synthase (iNOS)-Loaded Mineralized Nanoparticles on Embryonic Stem Cells: J.S. Lee, et al.; Cell. Physiol. Biochem. 51, 746 (2018), Abstract
Leukemia inhibitory factor reduces body fat mass in ovariectomized mice: J.O. Jansson, et al.; Eur. J. Endocrinol. 154, 349 (2006), Abstract
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells: C.C. Shih, et al.; Blood 95, 1957 (2000), Abstract
Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells: R.L. Williams, et al.; Nature 336, 684 (1988), Abstract

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