Highly sensitive LH ELISA kit enabling detection of LH in serum, plasma and tissue culture media in just 2 hours.
- Sensitive measurement of LH, detecting as little as 5.2 mIU/ml
- Negligible reactivity from similar glycoprotein hormones
- High throughput format with results in 2 hours for up to 39 samples in duplicate
- Fully quantitative results that surpass semi-quantitative Western blot analysis
The LH ELISA kit is a colorimetric competitive enzyme immunoassay kit with results in 2 hours.
LH is produced by the gonadotroph cells in the anterior pituitary gland. It is responsible for ovulation in women and controls testosterone synthesis in men. An acute rise of LH, referred to as the “LH surge”, triggers ovulation and development of the corpus luteum in females. After ovulation, LH supports the transient life span of the corpus luteum acting on the luteinized granulosa cells. Cell enlargement and increased progesterone production are involved in the luteinization of the granulosa cells, which are closely associated with the developing gamete (egg) in the ovary.
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Product Details
Alternative Name |
Luteinizing hormone, Lutropin |
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Application |
Colorimetric detection, ELISA |
Application Notes |
For the quantitative determination of LH in tissue culture media, serum and plasma in human and rat samples. |
Assay Time |
2 hours |
Compatibility |
This product is compatible with the Absorbance 96 Plate Reader. |
Contents |
Microtiter Plate, Assay Buffer 13, Standard, Wash Buffer Concentrate, Antibody, Tracer, Conjugate, Substrate, and Stop Solution |
Crossreactivity |
LH (100%), FSH (≤0.004%), hCG (≤0.004%), TSH (≤0.3%) |
Sensitivity |
5.2 mIU/ml (1.2-280 mIU/ml) |
Species Reactivity |
Human, Rat |
UniProt ID |
P01215 (α chain), P01229 (β chain) |
Wavelength |
450 nm |
Handling & Storage
Long Term Storage |
+4°C |
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Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- Characterization of sperm motility and testosterone secretion in the taiep myelin mutant, a model of demyelination: L.P. Muñoz de la Torre, et al.; Anim. Reprod. 20, e20220102 (2023), Abstract
- Perfluorooctane sulfonic acid modulates expression of placental steroidogenesis-associated genes and hormone levels in pregnant rats: S.V. Dangudubiyyam, et al.; Reprod. Toxicol. 118, 108390 (2023), Application(s): Rat plasma, Abstract
- Sex-specific hypothalamic expression of kisspeptin, gonadotropin releasing hormone, and kisspeptin receptor in progressive demyelination model: L.P.M. de la Torre, et al.; J. Chem. Neuroanat. 123, 102120 (2022), Abstract
- Deficiency of Wnt10a causes female infertility via the β-catenin/Cyp19a1 pathway in mice: J.H. Zhang, et al.; Int. J. Med. Sci. 19, 701 (2022), Abstract
- Irisin ameliorates male sexual dysfunction in paroxetine-treated male rats: S. Canpolat, et al.; Psychoneuroendocrinology 136, 105597 (2021), Abstract
- Tri-ortho-cresyl phosphate exposure leads to low egg production and poor eggshell quality via disrupting follicular development and shell gland function in laying hens: J. Li, et al.; Ecotoxicol. Environ. Saf. 225, 112771 (2021), Abstract
- Increased testosterone and proinflammatory cytokines in patients with polycystic ovary syndrome correlate with elevated GnRH receptor autoantibody activity assessed by a fluorescence resonance energy transfer-based bioassay: H. Li, et al.; Endocrine 74, 163 (2021), Abstract
- The Role of GnRH Receptor Autoantibodies in Polycystic Ovary Syndrome: Kem, D. C., Li, H., et al.; J. Endocr. Soc. 4, bvaa078 (2020), Abstract
- Diet during early life defines testicular lipid content and sperm quality in adulthood: Crisóstomo, L., Videira, R. A., et al.; Am. J. Physiol. Endocrinol. Metab. 319, E1061 (2020), Abstract
- Downregulation of testosterone production through luteinizing hormone receptor regulation in male rats exposed to 17α-ethynylestradiol: P.H. Lin, et al.; Sci. Rep. 10, 1576 (2020), Abstract — Full Text
- Samul-tang ameliorates oocyte damage due to cyclophosphamide-induced chronic ovarian dysfunction in mice: J. Kim, et al.; Sci. Rep. 10, 21925 (2020), Abstract — Full Text
- Autoimmune activation of the GnRH receptor induces insulin resistance independent of obesity in a female rat model: H. Li, et al.; Physiol. Rep. 8, e14672 (2020), Abstract — Full Text
- microRNA expression profiles in the ventral hippocampus during pubertal development and the impact of peri-pubertal binge alcohol exposure: A. Asimes, et al.; Noncoding RNA 5, 21 (2019), Application(s): ELISA using rat plasma, Abstract — Full Text
- Chronic intermittent hypoxia induces hormonal and male sexual behavioral changes: Hypoxia as an advancer of aging: E.N. Wilson, et al.; Physiol. Behav. 189, 64 (2018), Application(s): ELISA using rat plasma, Abstract — Full Text
- Binge drinking and intergenerational implications: Parental preconception alcohol impacts offspring development in rats: A. Asimes, et al.; J. Endocr. Soc. 2, 672 (2018), Application(s): ELISA using rat plasma, Abstract — Full Text
- Welsh Onion Root (Allium fistulosum) Restores Ovarian Functions from Letrozole Induced-Polycystic Ovary Syndrome: Y.H. Lee, et al.; Nutrients 10, 1430 (2018), Application(s): Rat serum analysis, Abstract — Full Text
- Restoration of Spermatogenesis Using a New Combined Herbal Formula of Epimedium koreanum Nakai and Angelica gigas Nakai in an Luteinizing Hormone-Releasing Hormone Agonist-Induced Rat Model of Male Infertility: H.J. Park, et al.; World J. Mens Health 35, 170 (2017), Application(s): Rat blood serum, Abstract — Full Text
- Morinda Officinalis Polysaccharides Stimulate Hypothalamic GnRH Secretion in Varicocele Progression: Z. Zhu, et al.; Evid. Based Complement. Alternat. Med. 2017, 9057959 (2017), Abstract — Full Text
- Di-(2-ethylhexyl) phthalate inhibits testosterone level through disturbed hypothalamic-pituitary-testis axis and ERK-mediated 5α-Reductase 2: M. Ha, et al.; Sci. Total Environ. 563, 566 (2016), Application(s): Serum LH levels were measured, Abstract
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