Leptin (mouse), ELISA kit

• Flexible – broad dynamic range suitable for many normal and pathological samples
• Sensitive – measure < 26 pg/ml mouse leptin
• Fast – fully quantitative results in just 3 hours from up to 39 samples in duplicate
• Pre-coated plates and ready-to-use reagents save you time

The Leptin (mouse), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. The broad dynamic range is suitable for many normal and pathological samples with sensitive quantification of < 26 pg/mL mouse leptin.

The adipokine leptin is a hormone secreted predominantly by adipose tissue that signals through leptin receptors in the hypothalamus to decrease appetite and increase energy expenditure. Binding of leptin to the long-form of the leptin receptor in the hypothalamus reduces neuropeptide Y (NPY) and agouti-related protein (AgRP) activity, while stimulating anorexigenic pro-opiomelanocortin (POMC) neuron activity to reduce appetite. In peripheral tissues, leptin antagonizes insulin signaling, increases fatty acid oxidation, decreases insulin production in pancreatic β cells, and promotes fertility. Its expression in adipocytes can be regulated in a paracrine fashion by other adipokines such as IL-6 (stimulation) and TNF-α (inhibition).

Leptin deficient mice display severe insulin resistance, obesity, and decreased fertility, all of which are reversible by administration of exogenous leptin. In contrast, elevated circulating levels of leptin are associated with increased obesity, indicative of an acclimated state of leptin resistance which is not well understood. Evidence also links leptin to cardiovascular disease, as it mediates numerous pro-inflammatory and pro-atherogenic effects such as increased endothelin-1 (ET-1) expression, accumulation of reactive oxygen species (ROS), secretion of monocyte chemoattractant protein-1 (MCP-1), and increased sympathetic vascular tone and blood pressure.