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The LDLR antibody is essential in studies related to lipid metabolism, cardiovascular diseases, and atherosclerosis.
Low-Density Lipoprotein Receptor (LDLR) is a cell surface receptor that plays a vital role in cholesterol metabolism by mediating the endocytosis of LDL particles.
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Flow cytometric analysis of Mouse LDLR expression on Raw264.7. Cells were stained with purified anti-Mouse LDLR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence staining of Mouse LDLR in raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse LDLR monoclonal antibody (1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-rabbit IgG secondary antibody(green), counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
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RUO – Research Use Only |
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