LDH Based Cell Cytotoxicity Assay

Cell death or cytotoxicity is classically evaluated by assessing the degree of plasma membrane damage. Lactate dehydrogenase (LDH) is an intracellular enzyme that is released into the extracellular medium upon cellular damage or lysis, making it a useful indicator of cytotoxic effects. AkrivisBio’s LDH Cytotoxicity Assay Kit is used to assess cell membrane integrity and measure cell death or cytotoxicity. The assay utilizes WST, a tetrazolium detection reagent to give a fast, highly sensitive measure of LDH release from damaged cells. The intensity of color correlates directly with the number of damaged cells.