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LC3, mAb (5F10)

NT-0231

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Product Details

Alternative Name	anti-MAP1LC3 MAb (5F10), anti-Microtubule-associated Protein 1 Light Chain 3B MAb (5F10), anti-ATG8 MAb (5F10), anti-Autophagy-related Protein 8 MAb (5F10), anti-AUT7 MAb (5F10), anti-CVT5 MAb (5F10)
Application	ICC, WB
Clone	5F10
Host	Mouse
Immunogen	Synthetic peptide corresponding to the N-terminus of LC3-B (microtubule-associated protein 1 light chain 3B).
Isotype	IgG1
Positive Control	Included (Prod. No. NT-0911 Neuro 2A, untreated).
Species Reactivity	Dog, Hamster, Human, Mouse, Rat
Specificity	Recognizes human, mouse, rat, dog and hamster LC3. Recognizes both forms of endogenous human LC3, the cytoplasmic LC3-I as well as the lipidated form generated during autophagosome and autophagolysosome formation, LC3-II. Immunocytochemical staining of cells with LC3(5F10) monoclonal antibody reveals the specific punctate distribution of endogenous LC3-II as a hallmark of autophagic activity.
Technical Info / Product Notes	For the Original Manufacturer’s Data Sheet please click here: Unlabelled; Biotin. Our product description may differ slightly from the Original Manufacturer’s Data Sheet.

Handling & Storage

Handling	After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Short Term Storage	+4°C
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only