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LAG-3 (human) recombinant monoclonal antibody (L4-PL33)

ENZ-ABS677

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The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36 kDa) and LAG-3V3 (D1-D3, 52 kDa). The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome. LAG-3 expression by T cell clones correlated with IFN-γ production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses.

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This antibody is covered by our Worry-Free Guarantee.

IHC of LAG-3 (human) recombinant monoclonal antibody (L4-PL33) (Prod. No. ENZ-ABS677) in human tonsil tissue (10x).

IHC of LAG-3 (human) recombinant monoclonal antibody (L4-PL33) (Prod. No. ENZ-ABS677) in human tonsil tissue (20x).

IHC of LAG-3 (human) recombinant monoclonal antibody (L4-PL33) (Prod. No. ENZ-ABS677) in human tonsil tissue (40x).

LAG-3 (human) recombinant monoclonal antibody (L4-PL33) Immunohistochemistry — Immunohistochemistry analysis of human tonsil tissue (20x) using LAG-3 (human) recombinant mAb (L4-PL33), (Prod. No. ENZ-ABS677), at a dilution of 1:50.

LAG-3 (human) recombinant monoclonal antibody (L4-PL33) Western blot — Western blot analysis of LAG-3 recombinant mAb (L4-PL33) (Prod. No. ENZ-ABS677) using different cell lysates and purified LAG-3 protein: Lane 1 and 2: MW markers, Lane 3: Jurkat, Lane 4: HeLa, Lane 5: Empty, Lane 6: THP-2, Lane 7: HDML2, Lane 8: Empty, Lane 9: LAG-3 (human):Fc (human), (recombinant) (Prod. No. ALX-522-078).

Product Details

Alternative Name	Lymphocyte activation gene-3, FDC protein, CD223
Application	ELISA, Flow Cytometry, ICC, IHC, IP, WB
Clone	L4-PL33
Formulation	Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Host	Mouse
Immunogen	Synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-terminal D1 domain of human LAG-3 (lymphocyte activation gene-3).
Isotype	IgG1κ
Purity Detail	Purified.
Recommendation Dilutions/Conditions	Immunohistochemistry (frozen sections, 1:150)Western Blot (5 µg/ml)Immunoprecipitation (10 µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from tissue culture supernatant.
Species Reactivity	Human
Specificity	Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of LAG-3.
UniProt ID	P18627
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Use/Stability	Stable for at least 6 months after receipt when stored as recommended.
Handling	Avoid freeze/thaw cycles.
Short Term Storage	+4°C
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

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Related Products

LAG-3 (human):Fc (human), (recombinant)

ALX-522-078

7 citations

Alternative Name	Lymphocyte activation gene-3, FDC protein, CD223
Purity	≥99% (SDS-PAGE)
Source	Produced in CHO cells. The sequence coding for the 4 extracellular Ig-like domains of human LAG-3 (D1-D4) is fused to the Fc portion of human IgG1.