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- Increased stability
- Enhanced immune activation
KillerTRAIL™ (soluble) (human), (recombinant) is an enhanced ligand that utilizes the proprietary Killer™ linker peptide that promotes trimerization to form a more stable oligomer.
It has been well established that signals transmitted via TNF ligands and receptors are critical in mediating inflammation, cancer, and immunity. This has led to drug development aiming to either reduce or enhance receptor response. One limitation in this process is the inherent instability of recombinant ligands and their inability to effectively mimic the natural clustering of TNF receptors on the cell surface required for immune activation. Oligomerization of TNF ligands such as KillerTRAIL™ has been shown to improve stability and significantly enhance immune activation compared to recombinant ligands alone.
TNF-related apoptosis-inducing ligand (TRAIL) is a protein ligand that induces the process of cell death called apoptosis.
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Figure: Specificity of KillerTRAIL™-induced apoptosis. Concentration dependent blocking of apoptosis induction of BJAB cells using MAb to TRAIL (human) (2E5) (Prod. No. ALX-804-296) reveals specificity of KillerTRAIL™-mediated killing.Method: Human BJAB cells were treated with KillerTRAIL™, Soluble (human) (recombinant) (at 300ng/ml) in the presence or absence of the indicated concentration of the MAb to TRAIL (human) (2E5) or a control IgG1 mouse monoclonal antibody. After 16 hours apoptosis was evaluated by forward/sideward scatter (FSC/SSC) combined with propidium iodide (PI) stain analysed by flow cytometry.”””,KillerTRAIL™ Protein (soluble) (human)
Figure: Schematic structure of human TRAIL (peptide aa 95-281).
Figure: Apoptosis of TRAIL-sensitive cells. Concentration dependence of apoptosis induction in Jurkat and BJAB cells by KillerTRAIL™, Soluble (human) (recombinant) (Prod. No. ALX-201-073) reveals high activity even at concentrations of 10-100ng/ml.Method: 5×104 human Jurkat (JK) or BJAB cells were cultured in 100µl RPMI 1640 medium containing 10% fetal calf serum for 16 hours at 37°C in a 96-well plate in the presence of the indicated concentrations of KillerTRAIL™. Concentrations of KillerTRAIL™ required to induce apoptosis may vary depending on the cell type studied. Cell death was quantified by forward/sideward scatter (FSC/SSC) combined with propidium iodide (PI) staining analysed by flow cytometry.”””,KillerTRAIL™ Protein (soluble) (human)
Figure: Apoptosis of TRAIL-sensitive myeloid cell line ML-1.
Figure: Flow cytometry analysis of the TRAIL-sensitive human leukemia cell line HL60 and of TRAIL-resistant K572 cells.
| Regulatory Status |
RUO – Research Use Only |
|---|
Related Products
SUPERKILLERTRAIL® Protein (soluble) (human), (recombinant)
ALX-201-115
Enhanced ligand with improved stability providing significantly enhanced immune activation.
| Alternative Name | TNFSF 10, Apo-2L, CD253 |
|---|---|
| Purity | ≥98% (SDS-PAGE). MS analysis, <1% impurity (mainly Hsp70 protein from E. coli) |
| Source | Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge. |
MEGACD40L® Protein (soluble) (human), (recombinant)
ALX-522-110
High activity, high purity CD40L protein for co-stimulatory activation of an immune response
| Alternative Name | CD40L:ACRP30headless, CD154:ACRP30headless, TNFSF 5:ACRP30headless, gp39:ACRP30headless |
|---|---|
| Purity | ≥90% (SDS-PAGE) |
| Source | Produced in CHO cells. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag. |
| Alternative Name | Apo-2L, TNFSF 10, CD253 |
|---|---|
| Application | Flow Cytometry, FUNC |
| Host | Mouse |
| Isotype | IgG1 |
| Species Reactivity | Human |