Keratinocyte Transglutaminase (human) (recombinant) (His)

TGase 1 (recombinant) (His), TG1 (recombinant) (His), Keratinocyte Protein-glutamine γ-Glutamyltransferase (recombinant) (His)

White solid.

Lyophilized from 1ml 50mM TRIS pH8, 10mM glutathione.

~90kDa

≥90% (SDS-PAGE)

Reconstitute with 10mM glutathione dissolved in water. For activation add 10mM Ca2+. After reconstitution, the solution should be cooled on ice for short term storage. Storage for several weeks is possible at ≤-20°C after addition of 50% glycerol.

Produced in E. coli.

>3000U/mg protein. One unit is defined as the increase in fluorescence intensity of 1a.u./min. Activity is determined by measuring the rate of fluorescence enhancement after TG1-catalyzed monodansylcadaverine-incorporation into N, N-dimethylated casein (L.Lorand, et al.; Anal. Biochem. 44, 231 (1971)).

For the Original Manufacturer’s data sheet please click here.