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This antibody is covered by our Worry-Free Guarantee.
![[K(Ac)40]α-Tubulin polyclonal antibody Western blot](https://www.enzo.com/wp-content/uploads/2023/01/BML-SA452_WB.jpg?w=289)
Treatment of A549 cells with the histone deacetylase inhibitor trichostatin A (TSA, 0.3 µM, 7 hr.) induces acetylation of alpha-tubulin-Lys40. Western blot analysis was performed with A549 cell extracts (100,000 cells per lane) from either vehicle treated (Control) or TSAtreated (+TSA) cells. All lanes were probed with a 1:1000 dilution of the primary antibody, anti-alpha-tubulin (K(Ac)40) (Prod. No. BML-SA452). For lanes 2 and 5 the primary antibody was preincubated with the immunogen peptide (Ac-QMPSD-K(Ac)-TIGGG[CNH2] and for lanes 3 and 6 it was preincubated with non-acetylated peptide Ac-QMPSDKTIGGG[C-NH2] (peptides: 0.5 µg/µl, antibody: 1:100 dilution, 37°C, 1hr.). Secondary antibody for all lanes was donkey anti-rabbit-AP (1:2000; Jackson Immunoresearch) and the nitrocellulose membrane was developed with BCIP/NBT.
![[K(Ac)40]α-Tubulin polyclonal antibody Western blot](https://www.enzo.com/wp-content/uploads/2023/01/BML-SA452_WB.jpg?w=289)
Product Details
Application |
WB |
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Application Notes |
Detects a band of ~50kDa by Western blot. |
Formulation |
Liquid. In PBS. |
Host |
Rabbit |
Immunogen |
Synthetic acetylated peptide (K(Ac)40) corresponding to aa 35-45 (Q35MPSD-K(Ac)-TIGGG45) of human α-tubulin. |
Recommendation Dilutions/Conditions |
Western Blot (1:1,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
Source |
Purified from rabbit serum. |
Species Reactivity |
Chicken, Primate, Rodent, Xenopus, Zebrafish |
UniProt ID |
Q71U36 (TUBA1A), P68363 (TUBA1B), P0DPH7 (TUBA3C) |
Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
Handling |
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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Long Term Storage |
-20°C |
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- A Structural Mechanism for Noncanonical GPCR Signal Transduction in the Hedgehog Pathway: Steiner, W. P., Iverson, N., et al.; bioRxiv , (2024)
- Channel Function of Polycystin-2 in the Endoplasmic Reticulum Protects against Autosomal Dominant Polycystic Kidney Disease: Padhy, B., Xie, J., et al.; J. Am. Soc. Nephrol. 33, 1501 (2022), Abstract
- Differences in Functional Expression of Connexin43 and NaV1.5 by Pan- and Class-Selective Histone Deacetylase Inhibition in Heart: X. Zhang, et al.; Int. J. Mol. Sci. 19, E2288 (2018), Application(s): WB, Abstract — Full Text
- Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched: Zhang, Y., Bulkley, D. P., et al.; Cell 175, 1352 (2018), Abstract
- Changes in cardiac Nav1.5 expression, function, and acetylation by pan-histone deacetylase inhibitors: Xu, Q., Patel, D., et al.; Am. J. Physiol. Heart Circ. Physiol. 311, H1139 (2016), Abstract
- Histone deacetylase inhibition reduces cardiac connexin43 expression and gap junction communication: Q. Xu, et al.; Front. Pharmacol. 4, 44 (2013), Application(s): Western blot, Abstract — Full Text
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