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Mitochondria dye
JC-10 is a derivative of JC-1 useful for determining mitochondrial membrane potential by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-10 accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (590 nm) in healthy cells. However, upon cell injury, as membrane potential decreases, JC-10 monomers are generated, resulting in a shift to green emission (525 nm). The principal advantages of JC-10 relative to JC-1 include improved solubility in aqueous media and an ability to detect subtler changes in mitochondrial membrane potential loss. JC-10 allows for both qualitative visualization, considering the shift from orange to green fluorescence emission, and quantitative detection, considering the fluorescence intensity ratio, of mitochondrial membrane potential changes. Wavelength Maxima: Excitation 510nm, Emission 525nm
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Product Details
| Alternative Name |
Enhanced JC-1 |
|---|---|
| MW |
~600 |
| Purity |
≥95% (HPLC) |
| Solubility |
Soluble in DMSO. |
| Technical Info / Product Notes |
This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
Handling & Storage
| Use/Stability |
Stable for at least one year after receipt when stored as recommended. |
|---|---|
| Handling |
Protect from light. Keep cool and dry. |
| Long Term Storage |
-20°C |
| Shipping |
Ambient Temperature |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Epigallocatechin Gallate in Camellia sinensis Ameliorates Skin Aging by Reducing Mitochondrial ROS Production: Park, J. H., Jeong, E. Y., et al.; Pharmaceuticals (Basel) 18, 612 (2025), Abstract
- Sauchinone Ameliorates Senescence Through Reducing Mitochondrial ROS Production: M.U. Kuk, et al.; Antioxidants 14, 259 (2025), Abstract
- Identification of senescence rejuvenation mechanism of Magnolia officinalis extract including honokiol as a core ingredient: Lee, Y. H., Jeong, E. Y., et al.; Aging (Albany NY) 17, 497 (2025), Abstract
- Cardiac Tyrosine 97 Phosphorylation of Cytochrome c Regulates Respiration and Apoptosis: Morse, P. T., Pasupathi, V., et al.; Int. J. Mol. Sci. 26, (2025), Abstract
- Mitochondrial transfer mediates endothelial cell engraftment through mitophagy: Lin, R. Z., Im, G. B., et al.; Nature 629, 660 (2024), Abstract
- Trigonelline is an NAD+ precursor that improves muscle function during ageing and is reduced in human sarcopenia: Membrez, M., Migliavacca, E., et al.; Nat. Metab. 6, 433 (2024), Abstract
- Senescence Rejuvenation through Reduction in Mitochondrial Reactive Oxygen Species Generation by Polygonum cuspidatum Extract: In Vitro Evidence: Yoon, J. H., Kim, Y. H., et al.; Antioxidants (Basel) 13, (2024), Abstract
- Prostate Cancer-Specific Lysine 53 Acetylation of Cytochrome c Drives Metabolic Reprogramming and Protects from Apoptosis in Intact Cells: Morse, P. T., Wan, J., et al.; Biomolecules 14, (2024), Abstract
- Identification of Cellular Isoschaftoside-Mediated Anti-Senescence Mechanism in RAC2 and LINC00294: Lee, Y. H., So, B. H., et al.; Molecules 29, (2024), Abstract
- ER calcium depletion as a key driver for impaired ER-to-mitochondria calcium transfer and mitochondrial dysfunction in Wolfram syndrome: Liiv, M., Vaarmann, A., et al.; Nat. Commun. 15, 6143 (2024), Abstract
- Oral fecal transplantation enriches Lachnospiraceae and butyrate to mitigate acute liver injury: C. J. Yang, et al.; Cell Rep. 43, 113591 (2024), Abstract
- Connexin 43 Regulates Intercellular Mitochondrial Transfer from Human Mesenchymal Stromal Cells to Chondrocytes: Irwin, R. M., Thomas, M. A., et al.; bioRxiv , (2024), Reactant(s): Human
- Connexin 43 regulates intercellular mitochondrial transfer from human mesenchymal stromal cells to chondrocytes.: Irwin, R. M., Thomas, M. A., et al.; Stem Cell Res. Ther. 15, 359 (2024), Reactant(s): Human, Abstract
- Protective effects of caffeine against palmitate-induced lipid toxicity in primary rat hepatocytes is associated with modulation of adenosine receptor A1 signaling: J.C. Arroyave-Ospina, et al.; Biomed. Pharmacother. 165, 114884 (2023), Abstract
- Acute cigarette smoke exposure leads to higher viral infection in human bronchial epithelial cultures by altering interferon, glycolysis and GDF15-related pathways: Y. Wang, et al.; Respir. Res. 24, 207 (2023), Abstract
- Cytochrome c lysine acetylation regulates cellular respiration and cell death in ischemic skeletal muscle: Morse, P. T., Pérez-Mejías, G., et al.; Nat. Commun. 14, 4166 (2023), Abstract
- YY2-DRP1 Axis Regulates Mitochondrial Fission and Determines Cancer Stem Cell Asymmetric Division.: Wei, M., Nurjanah, U., et al.; Adv. Sci. (Weinh.) 10, e2207349 (2023), Application(s): FC/FACS / Reactant(s): Human, Abstract
- Protective effects of caffeine against palmitate-induced lipid toxicity in primary rat hepatocytes is associated with modulation of adenosine receptor A1 signaling.: Arroyave-Ospina, J. C., Buist-Homan, M., et al.; Biomed. Pharmacother. 165, 114884 (2023), Reactant(s): Rat, Abstract
- Methods for mitochondrial health assessment by High Content Imaging System: Panusatid, C., Thangsiriskul, N., et al.; MethodsX 9, 101685 (2022), Abstract
- TBC1D21 is an essential factor for sperm mitochondrial sheath assembly and male fertility: Y. Chen, et al.; Biol. Reprod. 107, 619 (2022), Abstract
- Reduced mitochondria membrane potential and lysosomal acidification are associated with decreased oligomeric Aβ degradation induced by hyperglycemia: A study of mixed glia cultures: Y. C. Huang, et al.; PLoS One 17, e0260966 (2022), Abstract
- In Vitro Effects of Paralytic Shellfish Toxins and Lytic Extracellular Compounds Produced by Alexandrium Strains on Hemocyte Integrity and Function in Mytilus edulis: Bianchi, V. A., Bickmeyer, U., et al.; Toxins (Basel) 13, (2021), Abstract
- RARG variant predictive of doxorubicin-induced cardiotoxicity identifies a cardioprotective therapy: Magdy, T., Jiang, Z., et al.; Cell Stem Cell 28, 2076 (2021), Abstract
- Effects of RAD51-stimulatory compound 1 (RS-1) and its vehicle, DMSO, on pig embryo culture: C.G. Lucas, et al.; Reprod. Toxicol. 105, 44 (2021), Abstract
- Elevated cAMP Protects against Diclofenac-Induced Toxicity in Primary Rat Hepatocytes: A Protective Effect Mediated by the Exchange Protein Directly Activated by cAMP/cAMP-Regulated Guanine Nucleotide Exchange Factors: Aguilar Mora, F. A., Musheshe, N., et al.; Mol. Pharmacol. 99, 294 (2021), Abstract
- Interference With Complex IV as a Model of Age-Related Decline in Synaptic Connectivity: M. Kriebel, et al.; Front. Mol. Neurosci. 13, 43 (2020), Abstract — Full Text
- Mitochondrial metabolic reprograming via BRAF inhibition ameliorates senescence: J.W. Kim, et al.; Exp. Gerontol. 126, 110691 (2019), Abstract
- Hypoxically Induced Nitric Oxide: Potential Role as a Vasodilator in Mytilus edulis Gills.: Abele, D., Rivera-Ingraham, G. A., et al.; Front. Physiol. 9, 1709 (2019), Abstract
- Human-Induced Pluripotent Stem Cell Model of Trastuzumab-Induced Cardiac Dysfunction in Patients With Breast Cancer: T. Kitani, et al.; Circulation 139, 2451 (2019), Abstract
- An aerobic eukaryotic parasite with functional mitochondria that likely lacks a mitochondrial genome: U. John, et al.; Sci. Adv. 5, eaav1110 (2019), Abstract — Full Text
- Spatial compartmentalization of free radical formation and mitochondrial heterogeneity in bivalve gills revealed by live-imaging techniques.: Meyer, S., Abele, D., et al.; Front. Zool. 13, 4 (2016), Abstract
- Degenerin channel activation causes caspase‐mediated protein degradation and mitochondrial dysfunction in adult C. elegans muscle: C.J. Gaffney, et al.; J. Cachexia Sarcopenia Muscle 7, 181 (2016), Application(s): Flow cytometry analysis of mitochondria membrane potential in C.elegans, Abstract — Full Text
- PINK1-dependent phosphorylation of PINK1 and Parkin is essential for mitochondrial quality control: N. Zhuang, et al.; Cell Death Dis. 7, e2501 (2016), Abstract — Full Text
- MKK3 influences mitophagy and is involved in cigarette smoke-induced inflammation: P. Mannam, et al.; Free Radic. Biol. Med. 101, 102 (2016), Application(s): Detecting Mitochondrial Membrane Potential (MMP) in BMDMs, Abstract
- PINK1 and Parkin cooperatively protect neurons against constitutively active TRP channel-induced retinal degeneration in Drosophila: Z. Huang, et al.; Cell Death Dis. 7, e2179 (2016), Application(s): Mitochondrial membrane potential was measured by staining with 25 μM JC-10 reagent, Abstract — Full Text
- Induced peroxidase and cytoprotective enzyme expressions support adaptation of HUVECs to sustain subsequent H₂O₂ exposure: H. Patel, et al.; Microvasc. Res. 2862, 30025 (2015), Application(s): Measument of HUVECs mitochondrial membrane polarization levels, Abstract
- 5,6-Dimethylxanthenone-4-acetic acid (DMXAA) activates stimulator of interferon gene (STING)-dependent innate immune pathways and is regulated by mitochondrial membrane potential: Prantner, D., Perkins, D. J., et al.; J. Biol. Chem. 287, 39776 (2012), Abstract
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