JBScreen Membrane 3 (Ammonium Sulfate/Alcohol/Salt based)

The JBScreen Membrane Screens 1-3 cover 72 of the most successful buffers for crystallization of membrane proteins. Their compositions result from analyzing the crystallization conditions of all membrane proteins crystallized so far.

The JBScreen Membrane crystallization buffers are principally ordered by type and concentration of the precipitant. Like in case of the “classic” JBScreen system, this allows easy extraction of all relevant information and is already a first step to a refinement: Once you get a hit, you immediately see the effects of the neighbouring conditions. Subsequent fine tuning of preliminary hits will be much more efficient.

The kit contains the following buffers:
A1 0.7 M Ammonium Sulfate, 1 M Na/K Phosphate pH 7.5
A2 1 M Ammonium Sulfate, 1 % Heptane-1,2,3-triol, 100 mM Zn Acetate, 50 mM Na MES pH 6.5
A3 1.2 M Ammonium Sulfate, 3 % Heptane-1,2,3-triol, 50 mM Tris HCl pH 7.5
A4 1.2 M Ammonium Sulfate, 100 mM Tris HCl pH 8.5
A5 1.4 M Ammonium Sulfate, 4 % iso-Propanol, 100 mM Ammonium Acetate
A6 2 M Ammonium Sulfate
B1 2 M Ammonium Sulfate, 100 mM Na Citrate pH 5.6
B2 2.5 M Ammonium Sulfate, 2 % PEG 5000 MME, 100 mM Na HEPES pH 7.5
B3 3 M Ammonium Sulfate, 3 % Heptane-1,2,3-triol
B4 3.5 M Ammonium Sulfate
B5 3.5 M Ammonium Sulfate, 250 mM Na Chloride, 50 mM Na/K Phosphate pH 7.5
B6 25 % MPD, 100 mM Bis-Tris pH 7.0
C1 30 % MPD, 300 mM Na Citrate pH 5.6
C2 25 % Triethylene Glycol, 100 mM Ammonium Sulfate, 100 mM Glycine
C3 30 % iso-Propanol, 20 % Glycerol, 200 mM Ca Chloride, 100 mM Na Acetate pH 4.6
C4 50 mM K Phosphate pH 8.0
C5 100 mM Na Citrate pH 4.8
C6 1 M K Phosphate pH 6.5, 3 % Heptane-1,2,3-triol, 1 % 1,4-Dioxane
D1 1 M Na Citrate
D2 1 M Na Citrate pH 5.6, 500 mM Li Chloride
D3 1.5 M Na Chloride, 100 mM Na Acetate pH 4.6
D4 1.5 M K Phosphate pH 7.0
D5 1.5 M Li Sulfate, 100 mM Na HEPES pH 7.5
D6 2 M Na Chloride, 100 mM Na Formate