JBScreen Classic 10 (Salt based)

The JBScreen Crystal Screening Kits 1-10 cover 240 of the most prominent buffers for protein crystallization. Their compositions result from data mining of several thousands of crystallized proteins. JBScreen represents the statistically most successful buffers that yielded protein crystals suitable for X-ray diffraction. The JBScreen buffers are principally ordered by type and concentration of the precipitant. This allows easy extraction of all relevant information and is already a first step to a refinement: Once you get a hit, you immediately see the effects of the neighbouring conditions. Subsequent fine tuning of preliminary hits will be much more efficient. Apart from saving you the tedious pipetting, sterile packed aliquots have several other important advantages: While common bulk solutions are frequently contaminated by the time you use the first aliquot, JBScreen is alalways fresh and sterile. Evaporation of solvent (with the resulting change of the buffer) just won’t happen – you will get extremely reproducible screening results.

The kit contains the following buffers:
A1 0.5 M Na Acetate, 0.1 M Imidazole HCl pH 8.0
A2 0.7 M Na Citrate, 0.1 M Na HEPES pH 7.5
A3 0.7 M Li Sulfate, 0.1 M TRIS HCl pH 8.5
A4 0.8 M K,Na Tartrate, 0.1 M Na HEPES pH 7.5
A5 1.0 M Ammonium Dihydrogen Phosphate, 0.1 M Na Citrate pH 5.6
A6 1.0 M Ammonium Dihydrogen Phosphate, 0.1 M TRIS HCl pH 8.5
B1 1.0 M Li Sulfate, 0.1 M TRIS HCl pH 8.5, 0.01 M Ni Chloride
B2 1.0 M Na Acetate, 0.1 M Imidazole HCl pH 8.0
B3 1.0 M Na Formate, 0.1 M Na Acetate pH 4.6
B4 1.4 M Na Acetate, 0.1 M Na MES pH 6.5
B5 1.4 M Na Citrate, 0.1 M Na HEPES pH 7.5
B6 1.5 M Li Sulfate, 0.1 M TRIS HCl pH 8.5
C1 1.5 M Na Citrate pH 6.5
C2 1.6 M Mg Sulfate, 0.1 M Na MES pH 6.5
C3 1.6 M K,Na Tartrate, 0.1 M Na MES pH 6.5
C4 2.0 M Ammonium Formate, 0.1 M Na MES pH 6.5
C5 2.0 M Ammonium Dihydrogen Phosphate, 0.1 M TRIS HCl pH 8.5
C6 2.0 M Na Formate
D1 2.0 M Mg Chloride, 0.1 M TRIS HCl pH 8.5
D2 2.0 M Na Chloride, 0.1 M Na MES pH 6.5, 0.2 M Na Acetate
D3 2.0 M Na Formate, 0.1 M Na Acetate pH 4.6
D4 1.0 M Ammonium Dihydrogen Phosphate, 30% Glycerol, 0.1 M TRIS HCl pH 8.5
D5 4.0 M Na Chloride, 0.1 M Na HEPES pH 7.5
D6 3.0 M Na Formate

Buffer preparation:
• Chemicals used were of MicroSelect grade for Molecular Biology.
• Buffers were prepared as 1 M stock solutions. The pH was adjusted with HCl (Imidazole, TRIS), NaOH (Bicine, CHES, HEPES, MES), Citric Acid (Citrate) or Acetic Acid (Acetate) (23°C; Fisher pH electrode).
• Percentages given are always w/v values (for solids as well as for liquids).
• The final volume was adjusted with >18 MOhm water.
• Solutions were filtered (0.2 µm) and filled in ampoules/tubes under sterile conditions.