JBScreen Basic HTS

JBScreen HTS is designed for high throughput crystallization applications using multi-channel pipets or crystallization robots. Each JBScreen HTS 96-well masterblock is pre-filled with 96 sterile crystallization buffers, with either 1.0 ml (S size) or 1.7 ml (L size) per well and is sealed with indexed cap mats.

Buffer preparation
• Chemicals used were of MicroSelect grade for Molecular Biology.
• Buffers were prepared as 1 M stock solutions. The pH was adjusted with HCl (Imidazole, TRIS), NaOH (Bicine, CHES, HEPES, MES), Citric Acid (Citrate) or Acetic Acid (Acetate) (23°C; Fisher pH electrode).
• Percentages given are always w/v values (for solids as well as for liquids).
• The final volume was adjusted with >18 MOhm water.
• Solutions were filtered (0.2 µm) and filled in ampoules/tubes under sterile conditions