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Interleukin 6 (IL-6) is an important pro-inflammatory and anti-inflammatory cytokine expressed by T cells, macrophages and muscle cells. IL-6 signals through a receptor complex containing two receptors, IL-6Rα and gp130. IL-6 has an important function in promoting fever and can serve to stimulate an immune response to trauma. IL-6 is often used for growth of hybridoma cell lines. Human IL-6 is active on mouse and rat cells.
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Figure 1: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Human IL-6 has a predicted MW of 20.9 kDa.
Figure 2: B9 cells were cultured with dilutions of Human IL-6. Cell proliferation can be measured and the linear portion of the curve can be used to calculate the ED50.
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RUO – Research Use Only |
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Last modified: May 29, 2024