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IL-35 (human):Fc (human), (recombinant)

ALX-522-140

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Highly pure interleukin involved in T cell regulation.

EBI3 (Epstein-Barr virus-induced gene 3 protein; EBV-induced gene 3 protein; IL-27B; IL-27 subunit β) and IL-12A (IL-12 subunit p35; CLMF p35) form the heterodimeric cytokine IL-35 (interleukin-35) involved in T cell regulation. IL-35 is highly expressed in regulatory T cell (Treg) but not in effector or naive T cells. IL-35 is required for the suppressive function of T-reg in vitro and in vivo.

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Product Details

Alternative Name	Interleukin-35, EBI3:IL-12A, IL-27B:CLMF p35
Concentration	0.1mg/ml after reconstitution.
Endotoxin Content	<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
Formulation	Lyophilized. Contains PBS.
MW	~85kDa
Purity	≥95% (SDS-PAGE)
Reconstitution	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Source	Produced in HEK 293 cells. Human EBI3 (aa 21-229) linked to human IL-12A (aa 23-219) via the linker GGGSGGGSGGGSGGGS is fused at the C-terminus to the Fc portion of human IgG1 and a FLAG^®-tag.
Technical Info / Product Notes	Historical data has shown that IL-35 binds to IL-12Rß2, and it has been demonstrated in the literature to inhibit mononuclear cell proliferation. FLAG is a registered trademark of Sigma-Aldrich Co.
UniProt ID	Q14213 (IL-27B), P29459 (IL-21A)

Handling & Storage

Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Handling	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Short Term Storage	+4°C
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Human IL-10-producing B cells have diverse states that are induced from multiple B cell subsets: Glass, M. C., Glass, D. R., et al.; Cell Rep. 39, 110728 (2022), Abstract
IL-27 Mediates PD-L1 Expression and Release by Human Mesothelioma Cells: Carbotti, G., Dozin, B., et al.; Cancers (Basel) 13, (2021), Abstract
Anti-inflammatory cytokines IL-35 and IL-10 block atherogenic lysophosphatidylcholine-induced, mitochondrial ROS-mediated innate immune activation, but spare innate immune memory signature in endothelial cells: Li, X., Fang, P., et al.; Redox Biol. 28, 101373 (2020), Abstract
Interleukin 35 Delays Hindlimb Ischemia-Induced Angiogenesis Through Regulating ROS-Extracellular Matrix but Spares Later Regenerative Angiogenesis: Fu, H., Sun, Y., et al.; Front. Immunol. 11, 595813 (2020), Abstract
IL-35 Inhibits Angiogenesis through VEGF/Ang2/Tie2 Pathway in Rheumatoid Arthritis: S. Jiang, et al.; Cell. Physiol. Biochem. 40, 1105 (2016), Application(s): Cell migration, In Vitro, Abstract
Pro-inflammatory effects of interleukin-35 in rheumatoid arthritis: M. Filková, et al.; Cytokine 73, 36 (2015), Application(s): Cell Culture: IHC, IF used to analyze IL-35 in PBMCs, Abstract
Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway: X. Sha, et al.; J. Biol. Chem. 290, 19307 (2015), Application(s): Cell culture, Abstract
IL-27 induces the expression of IDO and PD-L1 in human cancer cells: G. Carbotti, et al.; Oncotarget 6, 43267 (2015), Abstract — Full Text
Interleukin-27 is a potent inhibitor of cis HIV-1 replication in monocyte-derived dendritic cells via a type I interferon-independent pathway: Q. Chen, et al.; PLoS One 8, e59194 (2013), Application(s): Treatment of monocytes-derived dendritic cells, Abstract — Full Text
Regulatory T-Cell (Treg) hybridoma as a novel tool to study Foxp3 regulation and Treg fate: R. Sharma, et al.; J. Autoimmun. 37, 113 (2011), Abstract

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IL-35 (human):Fc (human), (recombinant)

ALX-522-140

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

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