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Figure: Immunohistochemical staining of endogenous human IL-33 in different human tissues (paraffin sections) using MAb to IL-33 (Nessy-1) (Prod. No. ALX-804-840). Method: Different human normal tissues (A: Stomach; B: Bronchus; C: Vulva/Anal skin; D: Nasopharynx) or cancer tissues (E: Colorectal cancer; F: Malignant glioma) were stained with MAb to lL-33 (Nessy-1) by standard immunohistochemistry. Pictures courtesy of Human Protein Atlas (http://www.proteinatlas.org/).”””””

Western Blot analysis of ALX-804-840: Lane 1: MW Marker, Lane 2: IL-33 (human), (recombinant), (Prod. No. ALX-522-098).

Expression of IL-33 and sST2 by the placenta.A) IL-33 was detected by western blotting of placental lysates from normal and pre-eclampsia placentas (n = 9). Both the full length (closed arrow) and cleaved forms (open arrow) were detected. ST2 was detected in each of the placental lysates. B) Densitometry of protein bands standardised to actin expression revealed no change in the levels of IL-33 nor sST2 between normal and pre-eclamptic placentas. C–H) Expression of IL-33 and sST2 can be seen in by immunocytochemical analysis of both normal (n = 3) and pre-eclampsia (n = 3) placentas (C-E and F-H show staining from two representative placentas respectively).
Image collected and cropped by CiteAb under a CC-BY license from the following publication: ST2 and IL-33 in pregnancy and pre-eclampsia. PLoS One (2011)

Expression of IL-33 and sST2 by the placenta.A) IL-33 was detected by western blotting of placental lysates from normal and pre-eclampsia placentas (n = 9). Both the full length (closed arrow) and cleaved forms (open arrow) were detected. ST2 was detected in each of the placental lysates. B) Densitometry of protein bands standardised to actin expression revealed no change in the levels of IL-33 nor sST2 between normal and pre-eclamptic placentas. C–H) Expression of IL-33 and sST2 can be seen in by immunocytochemical analysis of both normal (n = 3) and pre-eclampsia (n = 3) placentas (C-E and F-H show staining from two representative placentas respectively).
Image collected and cropped by CiteAb under a CC-BY license from the following publication: ST2 and IL-33 in pregnancy and pre-eclampsia. PLoS One (2011)




Product Details
Alternative Name |
Interleukin-33, IL-1F11, NF-HEV |
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Application |
ELISA, IHC (PS), IP, WB |
Clone |
Nessy-1 |
Formulation |
Liquid. In PBS containing 0.02% sodium azide. |
Gene/Protein Identifier |
90865 (Entrez GeneID), 608678 (OMIM) |
Host |
Mouse |
Immunogen |
Recombinant human IL-33 (aa 112-270) (Prod. No. ALX-522-098). |
Isotype |
IgG1κ |
Purity Detail |
Protein G-affinity purified. |
Recommendation Dilutions/Conditions |
Immunoprecipitation (1:100)Western Blot (1:1,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
Source |
Purified from ascites. |
Species Reactivity |
Human, Mouse, Rat |
Specificity |
Recognizes pro-IL-33 (precursor) and mature IL-33. |
Technical Info / Product Notes |
Cited samples: |
UniProt ID |
O95760 |
Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. |
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Short Term Storage |
+4°C |
Long Term Storage |
-20°C |
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- Endogenous IL-33 Accelerates Metacestode Growth during Late-Stage Alveolar Echinococcosis: B. Autier, et al.; Microbial Spectr. 11, e0423922 (2023), Abstract
- Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion: Chen, W., Chen, S., et al.; Nat. Immunol. 23, 1021 (2022), Abstract
- IL-33/ST2 receptor-dependent signaling in the development of pulmonary hypertension in Sugen/hypoxia mice: C.S. Indralingam, et al.; Physiol. Rep. 1481, e15185 (2022), Application(s): WB / Reactant(s) Mouse, Abstract
- Allergen protease-induced Gsdmd p40 controls IL-33 secretion: Sun, B., Wu, B., et al.; Research Square , (2021)
- A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis.: Hong, M., Sun, Y., et al.; Cell Death Dis. 12, 871 (2021), Application(s): IHC, Abstract
- Legumain promotes tubular ferroptosis by facilitating chaperone-mediated autophagy of GPX4 in AKI.: Wu, Y., Zhao, Y., et al.; Cell Death Dis. 12, 65 (2021), Application(s): WB, Abstract
- Protective role of IL33 signaling in negative pregnancy outcomes associated with lipopolysaccharide exposure.: Kozai, K., Iqbal, K., et al.; FASEB J. 35, e21272 (2021), Application(s): WB, Abstract
- NETs decorated with bioactive IL-33 infiltrate inflamed tissues and induce IFN-α production in patients with SL: S. Georgakis, et al.; JCI Insight 6, 147671 (2021), Application(s): ICC-IF / Reactant(s) Human, Abstract
- Immunomodulatory role of Interleukin-33 in large vessel vasculitis: A.C. Desbois, et al.; Sci. Rep. 10, 6405 (2020), Abstract — Full Text
- TLR3/TAK1 signalling regulates rhinovirus-induced interleukin-33 in bronchial smooth muscle cells: S. Ramu, et al.; ERJ. Open Res. 6, 147 (2020), Abstract — Full Text
- Staphylococcus aureus second immunoglobulin-binding protein drives atopic dermatitis via IL-33: A. Al Kindi, et al.; J. Allergy Clin. Immunol. S0091, 6749 (2020), Abstract
- Anacardic acid induces IL-33 and promotes remyelination in CNS: A. Ljunggren-Rose, et al.; PNAS 117, 21527 (2020), Abstract
- Cigarette Smoke Triggers IL-33-Associated Inflammation in a Model of Late Stage COPD: J.H. Lee, et al.; Am. J. Respir. Cell Mol. Biol. 61, 567 (2019), Abstract — Full Text
- Interleukin‑33 expression in ovarian cancer and its possible suppression of peritoneal carcinomatosis: A. Sekiya, et al.; Int. J. Oncol. 55, 755 (2019), Application(s): WB, Abstract
- Chronic exposure to high fat diet triggers myelin disruption and interleukin-33 upregulation in hypothalamus: H.T Huang, et al.; BMC Neurosci. 20, 33 (2019), Application(s): WB / Reactant(s) Mouse, Abstract — Full Text
- IL-33 drives influenza-induced asthma exacerbations by halting innate and adaptive antiviral immunity: L. Ravanetti, et al.; J. Allergy Clin. Immunol. 143, 1355 (2019), Abstract
- Stromal cells maintain immune cell homeostasis in adipose tissue via production of interleukin-33: Mahlakõiv, T., Flamar, A. L., et al.; Sci. Immunol. 4, (2019), Abstract
- Interleukin-33 is activated by allergen- and necrosis-associated proteolytic activities to regulate its alarmin activity during epithelial damage.: Scott, I. C., Majithiya, J. B., et al.; Sci. Rep. 8, 3363 (2018), Application(s): ICC / Reactant(s): Mouse, Abstract
- Decreased IL-33 Production Contributes to Trophoblast Cell Dysfunction in Pregnancies with Preeclampsia.: Chen, H., Zhou, X., et al.; Mediators Inflamm. 2018, 9787239 (2018), Reactant(s): Human, Abstract
- Chromatin regulates IL-33 release and extracellular cytokine activity.: Brusilovsky, M., Rymer, J. K., et al.; Nat. Commun. 9, 3244 (2018), Application(s): IF, Abstract
- A novel stromal lncRNA signature reprograms fibroblasts to promote the growth of oral squamous cell carcinoma via LncRNA-CAF/interleukin-33: Ding, L., Ren, J., et al.; Carcinogenesis 39, 397 (2018), Abstract
- Alarmins in Frozen Shoulder: A Molecular Association Between Inflammation and Pain.: Millar, N. L., Kerr, S. C., et al.; Am. J. Sports Med. 46, 671 (2018), Application(s): IHC / Reactant(s): Human, Abstract
- Extracellular Neutrophil Proteases Are Efficient Regulators of IL-1, IL-33, and IL-36 Cytokine Activity but Poor Effectors of Microbial Killing: Clancy, D. M., Sullivan, G. P., et al.; Cell Rep. 22, 2937 (2018), Abstract
- Interleukin-33 Predicts Poor Prognosis and Promotes Renal Cell Carcinoma Cell Growth Through its Receptor ST2 and the JNK Signaling Pathway: C.W. Wu, et al.; Cell. Physiol. Biochem. 47, 191 (2018), Abstract — Full Text
- Immunopathological Features of Severe Chronic Atopic Keratoconjunctivitis and Effects of Topical Cyclosporine Treatment: C.A. Utine, et al.; Ocul. Immunol. Inflamm. 6, 1 (2018), Abstract
- Full Length Interleukin 33 Aggravates Radiation-Induced Skin Reaction: Kurow, O., Frey, B., et al.; Front. Immunol. 8, 722 (2017), Abstract
- IL-33 is induced in undifferentiated, non-dividing esophageal epithelial cells in eosinophilic esophagitis: Travers, J., Rochman, M., et al.; Sci. Rep. 7, 17563 (2017), Abstract
- Interleukin-33 regulates tissue remodelling and inhibits angiogenesis in the eye: S. Theodoropoulou, et al.; J. Pathol. 241, 45 (2017), Application(s): IHC / Reactant(s) Mouse, Abstract — Full Text
- A novel isoform of IL-33 revealed by screening for transposable element promoted genes in human colorectal cancer: F.E. Lock, et al.; PLoS One 12, e0180659 (2017), Application(s): WB, Abstract — Full Text
- IL33 Promotes Colon Cancer Cell Stemness via JNK Activation and Macrophage Recruitment: M. Fang, et al.; Cancer Res. 77, 2735 (2017), Abstract — Full Text
- Ovatodiolide suppresses allergic airway inflammation and hyperresponsiveness in a murine model of asthma: C.N. Wang, et al.; Eur. J. Pharmacol. 812, 9 (2017), Abstract
- Elevated IL-33 expression is associated with pediatric eosinophilic esophagitis, and exogenous IL-33 promotes eosinophilic esophagitis development in mice: Judd, L. M., Heine, R. G., et al.; Am. J. Physiol. Gastrointest. Liver Physiol. 310, G13 (2016), Abstract
- Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation: Stojkovic, S., Kaun, C., et al.; Sci. Rep. 6, 25171 (2016), Abstract
- Loss of interleukin 33 expression in colonic crypts – a potential marker for disease remission in ulcerative colitis: M.D. Gundersen, et al.; Sci. Rep. 6, 35403 (2016), Application(s): Immunostaining, paraffin-embedded biopsies , Abstract — Full Text
- Extracellular IL-33 cytokine, but not endogenous nuclear IL-33, regulates protein expression in endothelial cells: V. Gautier, et al.; Sci. Rep. 6, 34255 (2016), Application(s): Western blot, Abstract — Full Text
- TLR3 Agonist Poly-IC Induces IL-33 and Promotes Myelin Repair: C. Natarajan, et al.; PLoS One 11, e0152163 (2016), Application(s): Western blot, Abstract — Full Text
- An antitumorigenic role for the IL-33 receptor, ST2L, in colon cancer: C. O’Donnell, et al.; Br. J. Cancer 114, 37 (2016), Abstract — Full Text
- Serglycin in Quiescent and Proliferating Primary Endothelial Cells: Reine, T. M., Vuong, T. T., et al.; PLoS One 10, e0145584 (2015), Abstract
- Rhinoviral stimuli, epithelial factors and ATP signalling contribute to bronchial smooth muscle production of IL-33: Calvén, J., Akbarshahi, H., et al.; J. Transl. Med. 13, 281 (2015), Abstract
- MicroRNA29a regulates IL-33-mediated tissue remodelling in tendon disease: N. L. Millar, et al.; Nat. Commun. 6, 6774 (2015), Application(s): Immunohistochemistry, Abstract — Full Text
- Interleukin-33 is released in spinal cord and suppresses experimental autoimmune encephalomyelitis in mice: H. Chen, et al.; Neuroscience 308, 157 (2015), Application(s): Western Blot, Abstract
- Nuclear IL-33 regulates soluble ST2 receptor and IL-6 expression in primary human arterial endothelial cells and is decreased in idiopathic pulmonary arterial hypertension: D. Shao, et al.; Biochem. Biophys. Res. Commun. 451, 8 (2014), Application(s): Immunohistochemistry using human paraffin-embedded lung tissue sections; Immunoprecipitation and Western blot using human pulmonary arterial endothelial cells (HPAECs) and lung microvascular endothelial cells (HLMVECs), Abstract
- The Experimentally Induced Psoriatic Lesion Associates with Rapid but Transient Decrease in Interleukin-33 Immunostaining in Epidermis: M.M. Suttle, et al.; Acta Derm. Venereol. 95, 536 (2014), Application(s): Immunocytochemistry using human keratinocytes, Abstract — Full Text
- Unique Temporal and Spatial Expression Patterns of IL-33 in Ovaries during Ovulation and Estrous Cycle Are Associated with Ovarian Tissue Homeostasis: C.I. Carlock, et al.; J. Immunol. 193, 161 (2014), Application(s): Western blot application, Abstract
- Interleukin-33 drives a proinflammatory endothelial activation that selectively targets nonquiescent cells.: Pollheimer, J., Bodin, J., et al.; Arterioscler. Thromb. Vasc. Biol. 33, e47 (2013), Application(s): IF, WB / Reactant(s): Human, Abstract
- Components of the interleukin-33/ST2 system are differentially expressed and regulated in human cardiac cells and in cells of the cardiac vasculature: Demyanets, S., Kaun, C., et al.; J. Mol. Cell. Cardiol. 60, 16 (2013), Abstract
- IL-33 is expressed in epithelia from patients with cystic fibrosis and potentiates neutrophil recruitment: L. Roussel, et al.; J. Allergy Clin. Immunol. 131, 913 (2013), Application(s): Immunohistochemistry using human lung tissue samples, Abstract
- The IL-33/ST2 axis is associated with human visceral leishmaniasis and suppresses Th1 responses in the livers of BALB/c mice infected with Leishmania donovani: Rostan, O., Gangneux, J. P., et al.; Mbio 4, e00383 (2013), Abstract
- Interleukin 33 as a Mechanically Responsive Cytokine Secreted by Living Cells: R. Kakkar, et al.; J. Biol. Chem. 287, 6941 (2012), Abstract — Full Text
- The alarmin IL-33 is a notch target in quiescent endothelial cells: Sundlisaeter, E., Edelmann, R. J., et al.; Am. J. Pathol. 181, 1099 (2012), Abstract
- Contribution of IL-33-activated type II innate lymphoid cells to pulmonary eosinophilia in intestinal nematode-infected mice: K. Yasuda, et al.; Proc. Natl. Acad. Sci. 109, 3451 (2012), Application(s): Immunoblotting analysis of the levels of IL-33 protein in the lungs from mice, Abstract — Full Text
- The Dual Function Cytokine IL-33 Interacts with the Transcription Factor NF-kappaB To Dampen NF-kappaB–Stimulated Gene Transcription: S. Ali, et al.; J. Immunol. 187, 1609 (2011), Abstract — Full Text
- IL-33 Exacerbates Acute Kidney Injury: A. Akcay, et al.; J. Am. Soc. Nephrol. 22, 2057 (2011), Application(s): IHC application with Kidney tissues , Abstract — Full Text
- Endogenous IL-33 enhances Th2 cytokine production and T-cell responses during allergic airway inflammation: J. Louten, et al.; Int. Immunol. 23, 307 (2011), Application(s): IHC application with murine lung tissue, Abstract — Full Text
- ST2 and IL-33 in pregnancy and pre-eclampsia.: Granne, I., Southcombe, J. H., et al.; PLoS One 6, e24463 (2011), Application(s): IHC, WB, Abstract
- Interleukin-33 Induces Expression of Adhesion Molecules and Inflammatory Activation in Human Endothelial Cells and in Human Atherosclerotic Plaques: S. Demyanets, et al.; Arterioscler. Thromb. Vasc. Biol. 31, 2080 (2011), Abstract — Full Text
- The IL1-like cytokine IL33 and its receptor ST2 are abnormally expressed in the affected skin and visceral organs of patients with systemic sclerosis: M. Manetti, et al.; Ann. Rheum. Dis. 69, 598 (2010), Abstract
- Increased expression of IL-33 in severe asthma: evidence of expression by airway smooth muscle cells: D. Préfontaine, et al.; J. Immunol. 183, 5094 (2009), Application(s): Immunocytochemistry application with bronchial tissue samples, Abstract — Full Text
- IL-33 amplifies the polarization of alternatively activated macrophages that contribute to airway inflammation: M. Kurowska-Stolarska, et al.; J. Immunol. 183, 64649 (2009), Application(s): Immunohistochemistry application with frozen sections of bronchial tissue, Abstract — Full Text
- The IL-1-like cytokine IL-33 is inactivated after maturation by caspase-1: C. Cayrol & J.-P. Girard; PNAS 106, 9021 (2009), Abstract — Full Text
- Interleukin-33 Is Biologically Active Independently of Caspase-1 Cleavage: D. Talabot-Ayer, et al.; J. Biol. Chem. 284, 19420 (2009), Abstract — Full Text
- Caspase-1, Caspase-8, and Calpain Are Dispensable for IL-33 Release by Macrophages: T. Ohno, et al.; J. Immunol. 183, 7890 (2009), Abstract — Full Text
- Nuclear Interleukin-33 Is Generally Expressed in Resting Endothelium but Rapidly Lost upon Angiogenic or Proinflammatory Activation: A.M. Küchler, et al.; Am. J. Pathol. 173, 1229 (2008), Abstract
Related Products

Alternative Name | Interleukin-33, NF-HEV, IL-1F11 |
---|---|
Purity | ≥95% (SDS-PAGE) |
Source | Produced in E. coli. Untagged human IL-33 (aa 112-270). |

Alternative Name | Interleukin-33, IL-1F11, NF-HEV |
---|---|
Application | ELISA, IHC (PS) |
Host | Mouse |
Isotype | IgG1κ |
Species Reactivity | Human, Mouse, Rat |

Alternative Name | Interleukin-33, IL-1F11, NF-HEV |
---|---|
Application | ELISA, IHC (PS), IP, WB |
Host | Mouse |
Isotype | IgG1κ |
Species Reactivity | Human, Mouse, Rat |
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