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IHC universal negative control reagent

ADI-950-231

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IHC universal negative control reagent is specifically designed to work with rabbit, mouse and goat antibodies. The reagent has been titered to work with polymer-based secondary systems in manual and automated IHC platforms.

Negative controls are used to determine the specificity of an antibody. No staining should be observed when the primary antibody is omitted. Minimal non-specific staining should be detected when using the IHC universal negative control reagent.

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Product Details

Formulation	Liquid. Negative control reagent- mixture of purified goat, mouse and rabbit immunoglobulins (IgGs).
Technical Info / Product Notes	Interpretation of the results will be the sole responsibility of the user. Source is from purified goat, mouse and rabbit immunoglobulins (IgGs) and total IgG concentration is 1.5 ug/ml.

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Topical ATP Application in the Peripheral Swallowing-Related Regions Facilitates Triggering of the Swallowing Reflex Involving P2X3 Receptors: Hossain, M. Z., Ando, H., et al.; Function (Oxf.) 6, zqaf010 (2025), Abstract
Pharmacological activation of transient receptor potential vanilloid 4 promotes triggering of the swallowing reflex in rats.: Hossain, M. Z., Ando, H., et al.; Front. Cell. Neurosci. 17, 1149793 (2023), Application(s): IHC / Reactant(s): Rat, Abstract
Extracellular matrix proteins (fibronectin, collagen III, and collagen I) immunoexpression in goat tuberculous granulomas (Mycobacterium caprae): C. Neila, et al.; Vet. Res. Commun. 46, 1147 (2022), Abstract
Dietary restriction and medical therapy drives PPARα-regulated improvements in early diabetic kidney disease in male rats: Martin, W. P., Nair, M., et al.; Clin. Sci. (Lond.) 136, 1485 (2022), Abstract
Medications Activating Tubular Fatty Acid Oxidation Enhance the Protective Effects of Roux-en-Y Gastric Bypass Surgery in a Rat Model of Early Diabetic Kidney Disease: Martin, W. P., Chuah, Y. H. D., et al.; Front. Endocrinol. (Lausanne) 12, 757228 (2022), Abstract
TRPA1s act as chemosensors but not as cold sensors or mechanosensors to trigger the swallowing reflex in rats: Hossain, M. Z., Ando, H., et al.; Sci. Rep. 12, 3431 (2022), Abstract
Natural infection of parvovirus in wild fishing cats (Prionailurus viverrinus) reveals extant viral localization in kidneys: C. Piewbang, et al.; PLoS One 16, 247266 (2021), Abstract
TRPV Subfamily (TRPV2, TRPV3, TRPV4, TRPV5, and TRPV6) Gene and Protein Expression in Patients With Ulcerative Colitis: J.J.T. Maurino, et al.; J. Immunol. Res. 2020, 2906845 (2020), Abstract — Full Text
Inflammatory chemokine profiles and their correlations with effector CD4 T cell and regulatory cell subpopulations in cutaneous lupus erythematosus: S. Mendez-Flores, et al.; Cytokine 119, 95 (2019), Abstract
Stereotactic Body Radiation and Interleukin-12 Combination Therapy Eradicates Pancreatic Tumors by Repolarizing the Immune Microenvironment: Mills, B. N., Connolly, K. A., et al.; Cell Rep. 29, 406 (2019), Abstract

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Datasheet

Specific Protocol

Use IHC universal negative control reagent in place of the primary antibody with a section to evaluate non-specific staining and allow better interpretation of staining results.

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SDS

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