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IgE (non-immune) (human)

BPD-DIA-HE1

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IgE comes from a monoclonal cell line. This antibody is well-suited as a standard in IgE-quantifying assays due to its very low batch-to-batch variation. Other applications include immunochemistry and cellular immunology research.

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IgE (non-immune) (human) SDS-PAGE — Figure: SDS-PAGE Analysis: MW marker (1), purified IgE (non-immune) (human) Protein (2) (185 kDa), and IgE, purified from serum from a myeloma patient (3).

Product Details

Alternative Name	Immunoglobulin E
Application Notes	Can be used as standard in IgE quantifying assays. Functional Application: cell stimulation.
Formulation	Liquid. In 0.01M phosphate buffer, pH 7.4, containing 0.15M sodium chloride.
Purity	≥90% (SDS-PAGE)
Purity Detail	Protein L purified. The original antibody-producing B cells were obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B. Although BPD-DIA-HE1 is purified from a monoclonal hybridoma line derived from the antibody-producing donor cell, it must be handled as potentially infectious as all human material. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from fetal bovine serum.
Source	Purified human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Short Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

IgE induces proliferation in human airway smooth muscle cells: role of MAPK and STAT3 pathways: N.S. Redhu, et al.; Allergy Asthma Clin. Immunol. 9, 41 (2013), Application(s): Stimulation of human airway smooth muscle (HASM) cell’s with IgE, Full Text
Stimulation of human airway smooth muscle (HASM) cell’s with IgE: J. Balhara; , (2012), (thesis), Application(s): Stimulation of human airway smooth muscle (HASM) cell’s with IgE , Full Text
Double expression of CD34 and CD117 on bone marrow progenitors is a hallmark of the development of functional mast cell of Callithrix jacchus (common marmoset): S. Nunomura, et al.; Int. Immunol. 24, 593 (2012), Application(s): Stimulation of mast cells (MC) with IgE, Abstract
Aspergillus oryzae lectin induces anaphylactoid oedema and mast cell activation through its interaction with fucose of mast cell-bound non-specific IgE: K. Yamaki, et al.; Scand. J. Immunol. 74, 445 (2011), Application(s): Investigation of Aspergillus oryzae involvement in allergic bronchopulmonary aspergillosis (ABPA), Abstract
Proinflammatory and Th2 cytokines regulate the high affinity IgE receptor (FcepsilonRI) and IgE-dependant activation of human airway smooth muscle cells: N.S. Redhu, et al.; PLoS One 4, e6153 (2009), Application(s): Stimulation of human bronchial/tracheal smooth muscle (B/TSM) cells with IgE, Abstract
The study of surface properties of an IgE-sensitive aptasensor using an acoustic method: M. Šnejdárková, et al.; Anal. Bioanal. Chem. 390, 1087 (2008), Abstract
Viability of allergy (IgE) detection using an alternative aptamer receptor and electrochemical means: K.I. Papamichael, et al.; Sensor Actuat. B-Chem. 121, 178 (2007), Full Text
Validation of peptide epitope microarray experiments and extraction of quality data: T. Nahtman, et al.; J. Immunol. Methods 328, 1 (2007), Application(s): Positive control in microarray technology, Abstract
Memory T cell proliferation in cow’s milk allergy after CD25+ regulatory T cell removal suggests a role for casein-specific cellular immunity in IgE-mediated but not in non-IgE-mediated cow’s milk allergy: G.B.G. Sletten, et al.; Int. Arch. Allergy Immunol. 142, 190 (2007), Abstract
IgE modulates neutrophil survival in asthma: role of mitochondrial pathway: A.S. Saffar, et al.; J. Immunol. 178, 2535 (2007), Application(s): Stimulation of neutrophils, Abstract

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IgE (non-immune) (human)

BPD-DIA-HE1

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

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