Human: Neuroblastoma HR Reporter Cell line (SH-SY5Y, Includes I-Sce1 Transfection) (50 assays)

This is a cell-based DNA Repair HR reporter that is hosted in human neuroblastoma line SH-SY5Y (or SH cells). The kit is designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, pathway, signaling or response genes) that affect or impact the process of HR. The kit uses GFP as an in vivo readout for the HR pathway in a neuro-derived line that can be differentiated. HR is initiated by transfection with an I-Sce1 Expression plasmid (included). Analysis of pooled transfected cells reveals GFP+ cells in 2-3d post-transfection. GFP+ cells have successfully completed HR and can be tracked with single cell resolution.