Human:HeLa NHEJ Reporter Cell Line (Includes I-Sce1 Transfection) (125 assays)

This is a HeLa cell-based reporter kit to analyze NHEJ in live cells. Specifically this kit will identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and other genes) that affect or impact NHEJ repair. The kit uses GFP as an in situ readout for NHEJ activity in a cell context. The NHEJ process is activated by transfecting with an I-Sce1 expression plasmid. A specific reporter based assay for NHEJ can be very beneficial for anti-cancer drug discovery projects, learning more about the process of NHEJ repair and establishing intersecting pathways, druggable pathway targets, aging and disease modeling in cells.