Human Blood B Booster Kit

Several approaches have been developed by scientists aiming to study the physiology of circulating B cells. They are generally limited both by the low number of B cells (8-10% of peripheral blood mononuclear cells) and by their low capacity to enter cell cycle. The most successful approaches of these last decades were the CD40 system, a culture technique allowing the long-term growth of B lymphocytes, and the B cell transformation using the Epstein-Barr virus (EBV). However, the efficiency of these methods remains limited, targeting only 1% (CD40) and 1/10⁶ (EBV) of the B lymphocytes. An improved method has recently been reported but targeting only a very restricted IgG⁺CD22⁺ B cell population. Dendritics has developed the KitHuBBB^® as an improvement of the above approaches, in order to target the largest B cell population from peripheral blood, thus offering the opportunity to pursue studies on immortalized B cells representative of the overall peripheral B repertoire. Under the best conditions, the kit HuBBB^® allows the immortalization of up to 20% of the starting B cell population.
The kit HuBBB^® was packaged so as to make it very easy to handle for the scientists ; the kit HuBBB^® contains all the reagents needed to obtain from 5.10⁴ to 2.10⁵ independent B cell clones from 10⁷ peripheral blood mononuclear cells using ten 96 wells culture plates. The resulting cells are available for a wide range of studies including genomic, transcriptomic and proteomic fields.