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HSP90 monoclonal antibody (3G3)

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Alternative Name	Heat shock protein 90
Application	IF, IHC, IP, WB
Clone	3G3
Formulation	Liquid. Contains no preservatives.
Host	Mouse
Immunogen	Purified mouse HSP90 (heat shock protein 90).
Isotype	IgM
Purity Detail	Ammonium sulfate/ascites suspension.
Recommendation Dilutions/Conditions	Immunofluorescence (1:200)Immunohistochemistry (1:200)Western Blot (1:500)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Chicken, Human, Mouse, Rainbow trout, Rat
UniProt ID	P07901 (HSP90α), P11499 (HSP90β)
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Use/Stability	Before use, centrifuge suspension (4’000 x g for 15 min.) and discard supernatant. Resuspend pellet in 200µl PBS, pH 7.2. If desired, add 0.05% sodium azide. Stable for 6 months after PBS resuspension.Removal of residual ammonium sulfate is not usually necessary for IP procedure; for other uses it may be removed by dialysis or equivalent method.
Handling	Avoid freeze/thaw cycles.
Short Term Storage	+4°C
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

The protein chaperone HSP90 can facilitate the divergence of gene duplicates: Lachowiec, J., Lemus, T., et al.; Genetics 193, 1269 (2013), Abstract
Evidence that the 90-kDa heat shock protein (HSP90) exists in cytosol in heteromeric complexes containing HSP70 and three other proteins with Mr of 63,000, 56,000, and 50,000: G.H. Perdew; J. Biol. Chem. 266, 6708 (1991), Abstract — Full Text

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