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Hsp60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. Hsp60 is the eukaryotic homolog of the E. coli GroEL protein, and forms a multimeric complex in the mitochondria with Hsp10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place. TRiC/CCT, a eukaryotic relative of Hsp60, is expressed in the cytosol and participates in the folding of actin and tubulin substrates, but lacks any association with an Hsp10-like co-factor.
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This antibody is covered by our Worry-Free Guarantee.
HSP60 monoclonal antibody (LK-2) Western blot, Immunohistochemistry, Flow Cytometry
Product Details
| Alternative Name |
Chaperonin 60, CPN60, HspD1, heat shock protein 60 |
|---|---|
| Application |
ELISA, Flow Cytometry, IHC, IP, WB |
| Application Notes |
Detects a band of ~60kDa by Western blot. |
| Clone |
LK-2 |
| Formulation |
Liquid. In PBS, pH 7.2, containing 0.1mM PMSF and 50% glycerol. |
| GenBank ID |
M34664 |
| Host |
Mouse |
| Immunogen |
Recombinant human HSP60. |
| Isotype |
IgG1 |
| Purity Detail |
Protein G affinity purified. |
| Recommendation Dilutions/Conditions |
Flow Cytometry (10µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Source |
Purified from ascites. |
| Species Reactivity |
Bovine, C. elegans, Chicken, Dog, Ehrlichia sennetsu, Guinea pig, Hamster, Human, Monkey, Mouse, Plant, Porcine, Rabbit, Rat |
| UniProt ID |
P10809 |
| Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
| Long Term Storage |
-20°C |
|---|---|
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- The boundary of life and death: changes in mitochondrial and cytosolic proteomes associated with programmed cell death of Arabidopsis thaliana suspension culture cells.: Schwarze, J., Carolan, J. C., et al.; Front. Plant Sci. 14, 1194866 (2023), Application(s): WB / Reactant(s): Arabidopsis thaliana, Abstract
- Plasticity-Related Gene 5 Is Expressed in a Late Phase of Neurodifferentiation After Neuronal Cell-Fate Determination: Gross, I., Brandt, N., et al.; Front. Cell. Neurosci. 16, 797588 (2022), Application(s): WB, Abstract
- ANGEL2 phosphatase activity is required for non-canonical mitochondrial RNA processing: P. Clemente, et al.; Nat. Commun. 13, 5750 (2022), Abstract
- The one-carbon pool controls mitochondrial energy metabolism via complex I and iron-sulfur clusters: Schober, F. A., Moore, D., et al.; Sci. Adv. 7, (2021), Abstract
- Physiological responses (Hsps 60 and 32, caspase 3, H2O2 scavenging, and photosynthetic activity) of the coral Pocillopora damicornis under thermal and high nitrate stresses: M. Thummasan, et al.; Mar. Pollut. Bull. 171, 112737 (2021), Abstract
- Helicobacter hepaticus is required for immune targeting of bacterial heat shock protein 60 and fatal colitis in mice.: Imhof, A., Krug, A., et al.; Gut Microbes 13, 1 (2021), Application(s): WB / Reactant(s): Mouse, Abstract
- Exploiting the HSP60/10 chaperonin system as a chemotherapeutic target for colorectal cancer.: Ray, A. M., Salim, N., et al.; Bioorg. Med. Chem. 40, 116129 (2021), Application(s): WB, Abstract
- i>Helicobacter hepaticus/i> as disease driver in a novel CD40-mediated model of colitis: Friedrich, V., Forné, I., et al.; bioRxiv , (2020)
- Defects in CISD-1, a mitochondrial iron-sulfur protein, lower glucose level and ATP production in Caenorhabditis elegans.: Hsiung, K. C., Liu, K. Y., et al.; Biomed. J. 43, 32 (2020), Application(s): WB, Abstract
- Distribution and Activity of Mitochondrial Proteins in Vascular and Avascular Retinas: Implications for Retinal Metabolism: Chidlow, G., Wood, J. P. M., et al.; Invest. Ophthalmol. Vis. Sci. 60, 331 (2019), Abstract
- The ARL2 GTPase regulates mitochondrial fusion from the intermembrane space.: Kahn, R. A., Newman, L. E., et al.; Cell Logist. 7, e1340104 (2017), Application(s): ICC-IF / Reactant(s): Mouse, Abstract
- The abundance of the ARL2 GTPase and its GAP, ELMOD2, at mitochondria are modulated by the fusogenic activity of mitofusins and stressors.: Kahn, R. A., Zhou, C., et al.; PLoS One 12, e0175164 (2017), Application(s): IF / Reactant(s): Green monkey, Human, Mouse, Abstract
- Plasmids for variable expression of proteins targeted to the mitochondrial matrix or intermembrane space.: Kahn, R. A., Newman, L. E., et al.; Cell Logist. 6, e1247939 (2017), Reactant(s): Human, Abstract
- The cellular stress response of the scleractinian coral Goniopora columna during the progression of the black band disease: D. Seveso, et al.; Cell Stress Chaperones 22, 225 (2017), Application(s): Western Blot, Abstract — Full Text
- Modulation of Hsp60 in response to coral brown band disease: D. Seveso, et al.; Mar. Environ. Res. 115, 15 (2017), Abstract
- Mitochondrial chaperone HSP-60 regulates anti-bacterial immunity via p38 MAP kinase signaling: Jeong, D. E., Lee, D., et al.; EMBO J. 36, 1046 (2017), Abstract
- Hsp60 expression profiles in the reef-building coral Seriatopora caliendrum subjected to heat and cold shock regimes: D. Seveso, et al.; Mar. Environ. Res. 119, 1 (2016), Application(s): Western Blot, Abstract
- TLR4 mutation and HSP60-induced cell death in adult mouse cardiac myocytes: J.P. Heiserman, et al.; Cell Stress Chaperones 20, 527 (2015), Reactant(s) Mouse, Abstract — Full Text
- Vitamin C and E supplementation hampers cellular adaptation to endurance training in humans: a double-blind, randomised, controlled trial.: Paulsen, G., Cumming, K. T., et al.; J. Physiol. 592, 1887 (2014), Application(s): WB / Reactant(s): Human, Abstract
- The susceptibility of corals to thermal stress by analyzing Hsp60 expression: D. Seveso, et al.; Mar. Environ. Res. 99, 69 (2014), Application(s): Western Blot, Abstract
- The ARL2 GTPase is required for mitochondrial morphology, motility, and maintenance of ATP levels: Newman, L. E., Zhou, C. J., et al.; PLoS One 9, e99270 (2014), Abstract
- HuR and the bioenergetic signature of breast cancer: a low tumor expression of the RNA-binding protein predicts a higher risk of disease recurrence: J. Cuezva, et al.; Carcinogenesis 29, 2053 (2008), Application(s): IP using human cell lysates, Abstract
- Developmental changes of heat-shock proteins in porcine testis by a proteomic analysis: S.Y. Huang, et al.; Theriogenology 64, 1940 (2005), Application(s): Western blot using porcine testis, Abstract
- Differential sublocalization of the dynamin-related protein OPA1 isoforms in mitochondria: H. Endo, et al.; Biochem. Biophys. Res. Commun. 300, 482 (2003), Application(s): WB using human samples, Abstract
- Human mitochondrial DNA is packaged with TFAM: D. Kang, et al.; Nucleic Acids Res. 31, 1640 (2003), Application(s): WB using human samples, Abstract
- Studies characterizing 60 kda autoantibodies in subjects with schizophrenia: M.H. Rapaport, et al.; Biol. Psychiatry 53, 361 (2003), Application(s): WB using human samples, Abstract
- Elevated humoral immune response to heat shock protein 60 (hsp60) family in periodontitis patients: K. Hara, et al.; Clin. Exp. Immunol. 120, 285 (2000), Application(s): WB using human samples, Abstract
- Plasma membrane expression of heat shock protein 60 in vivo in response to infection: S.R. Carding, et al.; Infect. Immun. 67, 4191 (1999), Application(s): IHC using mouse samples, Abstract
- Vaginal heat shock protein expression in symptom-free women with a history of recurrent vulvovaginitis: S.S. Witkin, et al.; Am. J. Obstet. Gynecol. 180, 524 (1999), Application(s): EIA using human samples, Abstract
- Ehrlichia sennetsu groE operon and antigenic properties of the GroEL homolog: Y. Rikihisa, et al.; FEMS Immunol. Med. Microbiol. 18, 39 (1997), Application(s): WB using Ehrlichia samples, Abstract
- Monoclonal antibodies raised against infectious haematopoietic necrosis virus (IHNV) G protein and a cellular 90 kDa protein neutralize IHNV infection in vitro: J.Y. Lee, et al.; J. Gen. Virol. 77, 1731 (1996), Application(s): Western blot analysis compared to 90 kDa protein, Abstract — Full Text
- The 60 kDa heat shock protein in human semen: relationship with antibodies to spermatozoa and Chlamydia trachomatis: S.S. Witkin, et al.; Hum. Reprod. 11, 2600 (1996), Application(s): EIA using human samples, Abstract
- Two monoclonal antibodies generated against human hsp60 show reactivity with synovial membranes of patients with juvenile chronic arthritis: W. van Eden, et al.; J. Exp. Med. 175, 1805 (1992), Application(s): IHC using human samples, Abstract
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