AMPIVIEW® RNA probes
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Product Details
| Alternative Name |
Heat shock protein 60 (bacterial) |
|---|---|
| Application |
ICC, IP, WB |
| Application Notes |
Detects a band of ~60kDa by Western blot. |
| Clone |
A57-B9 |
| Crossreactivity |
Minimally cross-reacts with Borrelia burgdorfi HSP60. Does not cross-react with E. coli GroEL or human HSP60. |
| Formulation |
Liquid. Ascites diluted in PBS containing 0.05% sodium azide. |
| Host |
Mouse |
| Immunogen |
Recombinant Chlamydia trachomatis HSP60 (heat shock protein 60). |
| Isotype |
IgG1 |
| Recommendation Dilutions/Conditions |
Immunocytochemistry (1:500)Western Blot (1:1,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Species Reactivity |
Chlamydia |
| Specificity |
Recognizes all three genera of Chlamydia HSP60. Epitope mapping studies suggest binding of aa 517-522. |
| UniProt ID |
P0C0Z7 |
| Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
| Handling |
Avoid freeze/thaw cycles. |
|---|---|
| Long Term Storage |
-20°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Recruitment of the cellular lipid transport protein CERT to C. psittaci inclusions regulates the timing of bacterial egress: Scholz, J., Holland, G., et al.; Sci. Rep. 15, 18241 (2025), Abstract
- Recruitment of the cellular lipid transport protein CERT toC. psittaciinclusions regulates the timing of bacterial egress: Scholz, J., Holland, G., et al.; bioRxiv , (2024), Reactant(s): Human
- The formation ofChlamydia-containing spheres, a novel non-lytic egress pathway of the zoonotic pathogenChlamydia psittaci: Scholz, J., Holland, G., et al.; bioRxiv , (2023)
- Chlamydia trachomatis inhibits apoptosis in infected cells by targeting the pro-apoptotic proteins Bax and Bak: C.W. Kontchou, et al.; Cell Death Differ. 29, 2046 (2022), Application(s): WB, Abstract
- A non-death function of the mitochondrial apoptosis apparatus in immunity: Brokatzky, D., Dörflinger, B., et al.; EMBO J. 38, (2019), Abstract
- Chlamydia trachomatis Prevents Apoptosis Via Activation of PDPK1-MYC and Enhanced Mitochondrial Binding of Hexokinase II: Al-Zeer, M. A., Xavier, A., et al.; EBioMedicine 23, 100 (2017), Abstract
- The cellular ceramide transport protein CERT promotes Chlamydia psittaci infection and controls bacterial sphingolipid uptake: Koch-Edelmann, S., Banhart, S., et al.; Cell. Microbiol. 19, (2017), Abstract
- Infection of epithelial cells with Chlamydia trachomatis inhibits TNF-induced apoptosis at the level of receptor internalization while leaving non-apoptotic TNF-signalling intact: C. Waguia Kontchou, et al.; Cell. Microbiol. 18, 1583 (2016), Abstract
- In contrast to Chlamydia trachomatis, Waddlia chondrophila grows in human cells without inhibiting apoptosis, fragmenting the Golgi apparatus, or diverting post-Golgi sphingomyelin transport.: Häcker, G., Nölke, T., et al.; Infect. Immun. 83, 3268 (2015), Application(s): ICC-IF / Reactant(s): Human, Abstract
- Golgi fragmentation and sphingomyelin transport to Chlamydia trachomatis during penicillin-induced persistence do not depend on the cytosolic presence of the chlamydial protease CPAF.: Häcker, G., Kretz, O., et al.; PLoS One 9, e103220 (2014), Application(s): WB / Reactant(s): Human, Abstract
- Chlamydia infection depends on a functional MDM2-p53 axis: González, E., Rother, M., et al.; Nat. Commun. 5, 5201 (2014), Abstract
- Improved plaque assay identifies a novel anti-Chlamydia ceramide derivative with altered intracellular localization: Banhart, S., Saied, E. M., et al.; Antimicrob. Agents Chemother. 58, 5537 (2014), Abstract
- Autophagy restricts Chlamydia trachomatis growth in human macrophages via IFNG-inducible guanylate binding proteins: Al-Zeer, M. A., Al-Younes, H. M., et al.; Autophagy 9, 50 (2013), Abstract
- Chlamydia trachomatis disturbs epithelial tissue homeostasis in fallopian tubes via paracrine Wnt signaling: Kessler, M., Zielecki, J., et al.; Am. J. Pathol. 180, 186 (2012), Abstract
- Perfluorocarbons decrease Chlamydophila pneumoniae-mediated inflammatory responses of rat type II pneumocytes in vitro.: Wissel, H., Rüdiger, M., et al.; Pediatr. Res. 60, 264 (2006), Application(s): WB, Abstract
- Monoclonal antibodies define genus-specific, species-specific and cross-reactive epitopes of the chlamydial 60-kilodalton heat shock protein (hsp60): Specific immunodetection and purification of chlam: Y. Yuang, et al.; Infect. Immun. 60, 2288 (1992), Abstract
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