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HSP47 monoclonal antibody (M16.10A1)

ADI-SPA-470

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ADI-SPA-470-J — 1 mg

$2,214.00

HSP47 monoclonal antibody (M16.10A1) quantity
ADI-SPA-470-D — 50 µg

$257.00

HSP47 monoclonal antibody (M16.10A1) quantity
ADI-SPA-470-F — 200 µg

$530.00

HSP47 monoclonal antibody (M16.10A1) quantity

Hsp47, also known as colligin and gp46, is an ER-resident collagen-specific stress glycoprotein involved in the synthesis and assembly of varoius collagens as a molecular chaperone. Hsp47 is a member of the serpin superfamily, but localizes to the ER via binding of its C-terminal RDEL sequence to KDEL receptors in the ER. Hsp47 binds procollagen co-translationally as it enters the ER, and dissociates prior to its secretion.

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This antibody is covered by our Worry-Free Guarantee.

HSP47 monoclonal antibody (M16.10A1) Immunohistochemistry — Immunohistochemistry analysis of paraffin-embedded tissue section of rat carotid artery 14 days after balloon-injury to the artery, stained using Hsp47 (Colligin) mAb (M16.10A1).

HSP47 monoclonal antibody (M16.10A1) Western blot — Western blot analysis of HSP47, mAb (M16.10A1) (Prod. no. ADI-SPA-470): Lane 1: Rat recombinant HSP47; Lane 2: Rat-2 (heat shocked); Lane 3: L929 (heat shocked); Lane 4: 3T3 (heat shocked); Lane 5: CHO (heat shocked); Lane 6: HeLa (heat shocked).

Product Details

Alternative Name	SerpinH1, Colligin, Gp46, Heat shock protein 47, Collagen-binding protein
Application	IHC, WB
Application Notes	Detects a band of ~47kDa by Western blot.
Clone	M16.10A1
Formulation	Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
GenBank ID	X61598
Host	Mouse
Immunogen	Native rat HSP47.
Isotype	IgG2bλ
Purity Detail	Protein G affinity purified.
Recommendation Dilutions/Conditions	Immunohistochemistry (1:50)Western Blot (1:1,000, ECL)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from ascites.
Species Reactivity	Bovine, Chicken, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
Technical Info / Product Notes	Cited samples: For an overview on cited samples please click here.
UniProt ID	P50454
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Microfocused Ultrasound With Visualization Induces Remodeling of Collagen and Elastin Within the Skin: Marquardt, K., Hartmann, C., et al.; J. Cosmet. Dermatol. 24, e16638 (2025), Abstract
CRTAP-Null Osteoblasts Have Increased Proliferation, Protein Secretion, and Skeletal Morphogenesis Gene Expression with Downregulation of Cellular Adhesion: Barnes, A. M., Mitra, A., et al.; Cells 14, 518 (2025), Abstract
Hsp47 promotes biogenesis of multi-subunit neuroreceptors in the endoplasmic reticulum: Wang, Y. J., Di, X. J., et al.; Elife 13, (2024), Abstract
Inhibition of heat shock protein 47 suppressed collagen production in Tenon’s capsule fibroblasts.: Wang, W., lI, H., et al.; Folia Histochem. Cytobiol. 61, 153 (2023), Application(s): WB / Reactant(s): Rat, Abstract
Dextromethorphan inhibits collagen transport in the endoplasmic reticulum eliciting an anti-fibrotic response inex-vivoandin vitromodels of pulmonary fibrosis: Khan, M. M., Żukowska, J., et al.; bioRxiv , (2023), Reactant(s): Human
Endoplasmic reticulum exit sites are segregated for secretion based on cargo size: Saxena, S., Foresti, O., et al.; bioRxiv , (2023), Reactant(s): Human
Absence of TRIC-B from type XIV Osteogenesis Imperfecta osteoblasts alters cell adhesion and mitochondrial function – A multi-omics study: Jovanovic, M., Mitra, A., et al.; Matrix Biol. 121, 127 (2023), Abstract
Pentagalloyl glucose-stabilized decellularized bovine jugular vein valved conduits as pulmonary conduit replacement: Sinha, D., Nagy-Mehesz, A., et al.; Acta Biomater. 170, 97 (2023), Abstract
Disorganization of basement membrane zone architecture causes impaired melanocyte inhabitation in vitiligo: Yang, F., Yang, L., et al.; bioRxiv , (2022), Application(s): IHC
Melanoma RBPome identification reveals PDIA6 as an unconventional RNA-binding protein involved in metastasis: N.M. Farràs, et al.; Nucleic Acids Res. 50, 8207 (2022), Application(s): ICC-IF / Reactant(s) Human, Abstract