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Cell permeable DNA dye
Hoechst 33342 is fluorescent probe useful for detecting DNA by fluorescence microscopy and flow cytometry. Hoechst 33342 may be used on live or fixed cells and is also applicable for cell cycle analysis and monitoring DNA condensation by flow cytometry. An additional ethyl group renders Hoechst 33342 more lipophilic, and thus more readily cell permeable than Hoechst 33258 . Exclusion of Hoechst 33342 dye is a characteristic common to stem cells, as well as chemotherapy-resistant cancer cells. Wavelength Maxima: Excitation 350nm, Emission 461nm
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Product Details
| Alternative Name |
2’-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5’-bi-1H-benzimidazole |
|---|---|
| Appearance |
Yellow solid. |
| CAS |
23491-52-3 |
| Formula |
C27H28N6O . 3HCl |
| MW |
561.9 |
| Purity |
≥95% (HPLC) |
| Solubility |
Soluble in water (50mg/ml) or DMSO (10mg/ml). |
| Technical Info / Product Notes |
This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. Application Note: |
Handling & Storage
| Use/Stability |
Stable for at least one year after receipt when stored as recommended. |
|---|---|
| Handling |
Protect from light. Keep cool and dry. |
| Long Term Storage |
-20°C |
| Shipping |
Ambient Temperature |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Inhibition of S6K lowers age-related inflammation and increases lifespan through the endolysosomal system: Zhang, P., Catterson, J. H., et al.; Nat. Aging 4, 491 (2024), Abstract
- Endothelial Jagged1 levels and distribution are post-transcriptionally controlled by ZFP36 decay proteins: Sunshine, H. L., Cicchetto, A. C., et al.; Cell Rep. 43, 113627 (2024), Abstract
- A programmable platform for probing cell migration and proliferation: J. Cwycyshyn, et al.; APL Bioeng. 8, 046106 (2024), Reactant(s): Human, Abstract
- ALDH2 dysfunction and alcohol cooperate in cancer stem cell enrichment: S. Flashner, et al.; Carcinogenesis 45, 95 (2024), Abstract
- The Changes in Mitochondrial Morphology and Physiology Accompanying Apoptosis in Galleria mellonella (Lepidoptera) Immunocompetent Cells during Conidiobolus coronatus (Entomophthorales) Infection: A. Kaczmarek, et al.; Int. J. Mol. Sci. 24, 10169 (2023), Abstract
- Validation of the volumetric flow cytometry for bovine sperm concentration: Gloria, A., Bracco, C., et al.; Research Square , (2023)
- Microfluidic coaxial 3D bioprinting of cell-laden microfibers and microtubes for salivary gland tissue engineering: Yin, Y., Vázquez-Rosado, E. J., et al.; Biomater. Adv. 154, 213588 (2023), Abstract
- ALDH2 dysfunction accelerates ESCC pathogenesis: Flashner, S., Shimonosono, M., et al.; bioRxiv , (2023)
- Automated In Vitro Wound Healing Assay: Cwycyshyn, J., Stansbury, C., et al.; bioRxiv , (2023), Reactant(s): Human
- Validation of the volumetric flow cytometry for bovine sperm concentration.: Gloria, A., Bracco, C., et al.; Sci. Rep. 13, 16560 (2023), Application(s): FC/FACS, Abstract
- Toward Smart Biomimetic Apatite-Based Bone Scaffolds with Spatially Controlled Ion Substitutions: E. Cianflone, et al.; Nanomaterials 13, 519 (2023), Abstract
- Parkin regulates neuronal lipid homeostasis through SREBP2-lipoprotein lipase pathway – implications for Parkinson’s disease: W. Tang, et al.; Hum. Mol. Genet. 32, 1466 (2023), Abstract
- In vivo optical imaging of tumor stromal cells with hypoxia-inducible factor activity: H. Miyabara, et al.; Cancer Sci. , (2023), Abstract
- ROS-mediated activation of p38 protects hepatocellular carcinoma cells from caspase-independent death elicited by lysosomal damage: S. Castelli, et al.; Biochem. Pharmacol. 198, 114983 (2022), Abstract
- Infection of Galleria mellonella (Lepidoptera) Larvae With the Entomopathogenic Fungus Conidiobolus coronatus (Entomophthorales) Induces Apoptosis of Hemocytes and Affects the Concentration of Eicosanoids in the Hemolymph: A.K. Wronska, et al.; Front. Physiol. 12, 774086 (2022), Application(s): Fluorescence Microscopy and Flow Cytometry of Insect Larval hemocytes, Abstract
- Alcohol Metabolism Enriches Squamous Cell Carcinoma Cancer Stem Cells That Survive Oxidative Stress via Autophagy: M. Shimonosono, et al.; Biomolecules 11, 1479 (2022), Abstract
- Inhibition of S6K lowers age-related inflammation and immunosenescence and increases lifespan through the endolysosomal system: Zhang, P., Catterson, J. H., et al.; bioRxiv , (2022)
- Pancreatic tumor microenvironmental acidosis and hypoxia transform gold nanorods into cell-penetrant particles for potent radiosensitization: P.R. Rauta, et al.; Sci. Adv. 8, eabm9729 (2022), Abstract
- Enhanced antibacterial activity of Rosehip extract-functionalized Mg(OH)2 nanoparticles: An in vitro and in vivo study: M.M. Alves, et al.; Colloids Surf. B Biointerfaces 217, 112643 (2022), Abstract
- Intracellular cytokine detection based on flow cytometry in hemocytes from Galleria mellonella larvae: A new protocol: A.K. Wrońska, et al.; PLoS One 17, e0274120 (2022), Abstract
- SARS-CoV2 infection impairs the metabolism and redox function of cellular glutathione: D. Bartolini, et al.; Redox Biol. 45, 102041 (2021), Abstract — Full Text
- Immunosuppressed Miniswine as a Model for Testing Cell Therapy Success: Experience With Implants of Human Salivary Stem/Progenitor Cell Constructs: Wu, D., Lombaert, I. M. A., et al.; Front. Mol. Biosci. 8, 711602 (2021), Abstract
- Gadolinium Labelled Nanoliposomes as the Platform for MRI Theranostics: In Vitro Safety Study in Liver Cells and Macrophages: P. Simeckova, et al.; Sci. Rep. 10, 4780 (2020), Abstract — Full Text
- Cardiac‐specific Mst1 deficiency inhibits ROS‐mediated JNK signalling to alleviate Ang II‐induced cardiomyocyte apoptosis: Z. Cheng, et al.; J. Cell. Mol. Med. 23, 543 (2019), Application(s): Microplate assay using mouse cardiomyocytes, Abstract — Full Text
- Perlecan domain I gradients establish stable biomimetic heparin binding growth factor gradients for cell migration in hydrogels: Hubka, K. M., Carson, D. D., et al.; Acta Biomater. 97, 385 (2019), Abstract
- Prenatal influenza vaccination rescues impairments of social behavior and lamination in a mouse model of autism: Y. Wu, et al.; J. Neuroinflammation 15, 228 (2018), Abstract — Full Text
- CD15 Expression Does Not Identify a Phenotypically or Genetically Distinct Glioblastoma Population: E. Kenney-Herbert, et al.; Stem Cells Transl. Med. 4, 822 (2018), Abstract — Full Text
- Targeting Orai1-mediated store-operated calcium entry by RP4010 for anti-tumor activity in esophagus squamous cell carcinoma: Cui, C., Chang, Y., et al.; Cancer Lett. 432, 169 (2018), Abstract
- FOXO3 is essential for CD44 expression in pancreatic cancer cells: M. Kumazoe, et al.; Oncogene 36, 2643 (2017), Abstract
- Development of alphaviral vectors for macrophage activation in cancer immunotherapy: B. Kurena; , (2016), (master’s thesis), Application(s): Cell immunostaining, Full Text
- Effects of mechanical stretching on the morphology and cytoskeleton of vaginal fibroblasts from women with pelvic organ prolapse: S. Wang, et al.; Int. J. Mol. Sci. 16, 9406 (2015), Application(s): Cell Culture, Microscopy, Abstract — Full Text
- Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells: J.H. Kim, et al.; Int. J. Nanomedicine 10, 5513 (2015), Application(s): Cell culture of human cells, Abstract — Full Text
- Utilization of fluorescent probes for the quantification and identification of subcellular proteomes and biological processes regulated by lipid peroxidation products: T.D. Cummins, et al.; Free Radic. Biol. Med. 59, 56 (2013), Application(s): Cell Culture, Abstract — Full Text
- Hormone-induced 14-3-3γ adaptor protein regulates steroidogenic acute regulatory protein activity and steroid biosynthesis in MA-10 Leydig cells: Y. Aghazadeh, et al.; J. Biol. Chem. 287, 15380 (2012), Application(s): Nuclear staining of Adult mouse testis sections, Abstract — Full Text
- Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D: I. Schmid, et al.; Nat. Protoc. 2, 187 (2007), Abstract
- High-throughput microtiter assay for Hoechst 33342 dye uptake: G.M. Seigel & L.M. Campbell; Cytotechnology 45, 155 (2004), Abstract
Related Products
| Appearance | Yellow solid. |
|---|---|
| Purity | ≥95% (HPLC) |
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