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Hoechst 33258 (ultra pure)

ENZ-52402

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DNA dye

Hoechst 33258 is a fluorescent probe useful for detecting DNA by fluorescence microscopy and flow cytometry. Hoechst 33258 may be used on live or fixed cells and is also applicable for cell cycle analysis and monitoring DNA condensation by flow cytometry. Hoechst 33358 is less cell permeable that other derivatives, however this dye also allows for quantitative measurements when plotted in a standard emission-to-content curve. Wavelength Maxima: Excitation 352nm, Emission 461nm

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Product Details

Alternative Name	4-[5-(4-Methyl-1-piperazinyl)[2,5′-bi-1H-benzimidazol]-2′-yl]-phenol . trihydrochloride
Appearance	Yellow solid.
CAS	23491-45-4
Formula	C₂₅H₂₄N₆O . 3HCl
MW	533.9 (trihydrochloride)
Purity	≥95% (HPLC)
Solubility	Soluble in DMSO.
Technical Info / Product Notes	This product is a member of the CELLESTIAL^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

Handling & Storage

Use/Stability	Stable for at least one year after receipt when stored as recommended.
Handling	Protect from light. Keep cool and dry.
Long Term Storage	-20°C
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

Otoprotective Effects of Fucoidan Reduce Cisplatin-Induced Ototoxicity in Mouse Cochlear UB/OC-2 Cells: C.Y. Hsieh, et al.; Int. J. Mol. Sci. 24, 3561 (2023), Abstract
Parkin regulates neuronal lipid homeostasis through SREBP2-lipoprotein lipase pathway – implications for Parkinson’s disease: W. Tang, et al.; Hum. Mol. Genet. 32, 1466 (2023), Abstract
The Changes in Mitochondrial Morphology and Physiology Accompanying Apoptosis in Galleria mellonella (Lepidoptera) Immunocompetent Cells during Conidiobolus coronatus (Entomophthorales) Infection: A. Kaczmarek, et al.; Int. J. Mol. Sci. 24, 10169 (2023), Abstract
Bisdemethoxycurcumin-mediated Attenuation of Apoptosis Prevents Gentamicin-induced Ototoxicity in Mouse Cochlear UB/OC-2 Cells: T.Y. Kang, et al.; In Vivo 36, 1095 (2022), Abstract
Intracellular cytokine detection based on flow cytometry in hemocytes from Galleria mellonella larvae: A new protocol: A.K. Wrońska, et al.; PLoS One 17, e0274120 (2022), Abstract
Single-cell template strand sequencing by Strand-seq enables the characterization of individual homologs: Sanders, A. D., Falconer, E., et al.; Nat. Protoc. 12, 1151 (2017), Abstract
Nordihydroguaiaretic acid (NDGA) and α-mangostin inhibit the growth of Mycobacterium tuberculosis by inducing autophagy: S. Guzman-Beltran, et al.; Int. Immunopharmacol. 31, 149 (2016), Application(s): Counter-staining, Abstract
Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPSC-CMs) to Monitor Compound Effects on Cardiac Myocyte Signaling Pathways.: Guo, L., Davis, M., et al.; Curr. Protoc. Chem. Biol. 7, 141 (2015), Reactant(s): Human, Abstract
Hormone-induced 14-3-3γ adaptor protein regulates steroidogenic acute regulatory protein activity and steroid biosynthesis in MA-10 Leydig cells: Y. Aghazadeh, et al.; J. Biol. Chem. 287, 15380 (2012), Application(s): Nuclear staining of Adult mouse testis sections, Abstract — Full Text
DNA condensation monitoring after interaction with hoechst 33258 by atomic force microscopy and fluorescence spectroscopy: M. Saito, et al.; J. Biochem. 136, 813 (2004), Abstract

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Hoechst 33342 is a cell-permeable DNA stain that produces bright blue fluorescence with low cytotoxicity – ideal for dynamic, high-contrast imaging in live-cell applications.

36 citations

Appearance	Yellow solid.
Purity	≥95% (HPLC)

Last modified: May 29, 2024

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Hoechst 33258 (ultra pure)

ENZ-52402

Product Details

Handling & Storage

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