Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; “free” iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5′- or 3′- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.
Shipping: Available products typically ship within 24/48h, via priority shipping.
Do you need support? Contact Customer Service or Technical Support.
Online Account
Access or Create Your Account
This antibody is covered by our Worry-Free Guarantee.

Western blot analysis: Lane 1: MW Marker, Lane 2: HO-1 Recombinant Rat Protein (Prod. No. ADI-SPP-730), Lane 3: Rat Liver Microsomes (Prod. No. ADI-LYT-RM100).

Flow cytometry analysis of human lung cancer A2 cells stained using isotype control antibody (left) and HO-1 (Hsp32) mAb (HO -1-2) (right).

Immunohistochemistry analysis of frozen human prostate section (stressed) stained using HO-1 (Hsp32) mAb (HO-1-2).

HO-1 is rapidly increased after haptoglobin and hemopexin infusion.(A and B) SS-mice (n = 3/group) were infused with vehicle or equimolar (1 μmol/kg) Hb, Hp, Hpx, Hb + Hp, or Hb + Hpx. Livers were removed and flash frozen 1 hour after infusion. Hepatic microsomes were used to assess heme oxygenase (HO) activity (A) via bilirubin production and protein expression (B) via immunoblot. Bars are means ± SD, **p < .01 versus vehicle or Hb. (C and D) SS-mice (n = 3/group) were untreated or infused with Hp or Hpx (1 μmol/kg) at baseline (time 0). Livers were removed and flash frozen 24, 48 or 72 hours after infusion. Hepatic microsomes were used to assess (C) HO activity and (D) HO-1 protein expression via immunoblot. Bars are means ± SD, *p < .05 and **p < .01 versus untreated SS-mice. (E and F) SS-mice (n = 3/group) were infused with vehicle or increasing doses (0.0156, 0.0625, 0.25 or 1.0 μmols/kg) of Hp or Hpx at baseline. Livers and kidneys were removed and flash frozen 24 hours after infusion. Hepatic (E) and kidney (F) microsomes were used to assess HO activity. Bars are means ± SD.
Image collected and cropped by CiteAb under a CC-BY license from the following publication: Haptoglobin and hemopexin inhibit vaso-occlusion and inflammation in murine sickle cell disease: Role of heme oxygenase-1 induction. PLoS One (2018)





Product Details
Alternative Name |
HMOX1, Hsp32, Heat shock protein 32, Heme oxygenase 1 |
---|---|
Application |
Flow Cytometry, IHC, WB |
Application Notes |
Detects a band of ~32kDa by Western blot. |
Clone |
HO-1-2 |
Formulation |
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
GenBank ID |
J02722 |
Host |
Mouse |
Immunogen |
Native rat HO-1. |
Isotype |
IgG2b |
Purity Detail |
Protein G affinity purified. |
Recommendation Dilutions/Conditions |
Flow Cytometry (1:100)Western Blot (1:1,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
Source |
Purified from ascites. |
Species Reactivity |
Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat |
UniProt ID |
P06762 |
Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
Long Term Storage |
-20°C |
---|---|
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
---|
- Biochemical Insights into the Effects of a Small Molecule Drug Candidate on Imatinib-Induced Cardiac Inflammation: Szabó, R., Börzsei, D., et al.; Int. J. Mol. Sci. 26, 6661 (2025), Abstract
- Nrf2 deficiency in muscle attenuates experimental autoimmune myositis-induced muscle weakness: Himori, K., Yamada, M., et al.; J. Physiol. 602, 6189 (2024), Abstract
- Muscle-derived IL-1β regulates EcSOD expression via the NBR1-p62-Nrf2 pathway in muscle during cancer cachexia: Yamada, M., Warabi, E., et al.; J. Physiol. 602, 4215 (2024), Abstract
- Muscle p62 stimulates the expression of antioxidant proteins alleviating cancer cachexia.: Yamada, M., Warabi, E., et al.; FASEB J. 37, e23156 (2023), Application(s): WB, Abstract
- Mitochondrial Dysfunction Involved in the Cytotoxicity of Tramadol in Human Endometrial Carcinoma Cells: L.C. Liu, et al.; Int. J. Mol. Sci. 24, 99 (2023), Abstract
- The H2S Donor Sodium Thiosulfate (Na2S2O3) Does Not Improve Inflammation and Organ Damage After Hemorrhagic Shock in Cardiovascular Healthy Swine.: Messerer, D. A. C., Gaessler, H., et al.; Front. Immunol. 13, 901005 (2022), Application(s): WB, Abstract
- Physiological responses (Hsps 60 and 32, caspase 3, H2O2 scavenging, and photosynthetic activity) of the coral Pocillopora damicornis under thermal and high nitrate stresses: M. Thummasan, et al.; Mar. Pollut. Bull. 171, 112737 (2021), Abstract
- Hormetic Effects of Binaphthyl Phosphonothioates as Pro-oxidants and Antioxidants: Y. Hirata, et al.; Chem. Res. Toxicol. 33, 2892 (2020), Abstract — Full Text
- A Potential Involvement of Anandamide in the Modulation of HO/NOS Systems: Women, Menopause, and Medical Cannabinoids.: Varga, C., Kupai, K., et al.; Int. J. Mol. Sci. 21, (2020), Reactant(s): Rat, Abstract
- Breast cancer cell debris diminishes therapeutic efficacy through heme oxygenase-1-mediated inactivation of M1-like tumor-associated macrophages.: Kim, S. H., Saeidi, S., et al.; Neoplasia 22, 606 (2020), Application(s): IF, Abstract
- Roles of JNK/Nrf2 Pathway on Hemin-Induced Heme Oxygenase-1 Activation in MCF-7 Human Breast Cancer Cells.: Kim, J. S., Park, K. H., et al.; Medicina (Kaunas) 56, (2020), Application(s): WB / Reactant(s): Human, Abstract
- Oral carbon monoxide therapy in murine sickle cell disease: Beneficial effects on vaso-occlusion, inflammation and anemia: J.D. Belcher, et al.; PLoS One 13, e0205194 (2018), Application(s): WB / Reactant(s) Mouse, Abstract — Full Text
- Haptoglobin and hemopexin inhibit vaso-occlusion and inflammation in murine sickle cell disease: Role of heme oxygenase-1 induction: J.D. Belcher, et al.; PLoS One 13, e0196455 (2018), Application(s): WB / Reactant(s) Mouse, Abstract — Full Text
- Control of Oxidative Stress and Inflammation in Sickle Cell Disease with the Nrf2 Activator Dimethyl Fumarate.: Zhang, P., Nguyen, J., et al.; Antioxid. Redox Signal. 26, 748 (2017), Application(s): WB / Reactant(s): Mouse, Abstract
- The cellular stress response of the scleractinian coral Goniopora columna during the progression of the black band disease: D. Seveso, et al.; Cell Stress Chaperones 22, 225 (2017), Application(s): Western Blot, Abstract — Full Text
- Sexual dimorphism in the hepatic protein response to a moderate trans fat diet in senescence-accelerated mice: S.A. Bloomer, et al.; Lipids Health Dis. 16, 243 (2017), Reactant(s) Mouse, Abstract — Full Text
- Hepatic Overexpression of Hemopexin Inhibits Inflammation and Vascular Stasis in Murine Models of Sickle Cell Disease: G.M. Vercellotti, et al.; Mol. Med. 22, 437 (2016), Application(s): WB, Abstract — Full Text
- Anti-Apoptotic Effects of 3,3′,5-Triiodo-L-Thyronine in the Liver of Brain-Dead Rats: R.A. Rebolledo, et al.; PLoS One 10, e0138749 (2015), Application(s): Immunohistochemistry in rats, Abstract — Full Text
- Localization of heat shock proteins in cerebral cortical cultures following induction by celastrol.: Chow, A. M., Brown, I. R., et al.; Cell Stress Chaperones 19, 845 (2014), Application(s): ICC, ICC-IF, WB / Reactant(s): Rat, Abstract
- N,N-diethyldithiocarbamate promotes oxidative stress prior to myelin structural changes and increases myelin copper content: W. Valentine, et al.; Toxicol. Appl. Pharmacol. 239, 71 (2009), Application(s): WB using rat cell lysates, Abstract
- Increased intestinal expression of heme oxygenase-1 and its localization in patients with ulcerative colitis: Y. Naito, et al.; J. Gastroenterol. Hepatol. 23, S229 (2008), Application(s): IHC using human tissue, Abstract
- Haem oxygenase-I gene transfer protects retinal ganglion cells from ischaemia/reperfusion injury: S. Juan, et al.; Clin. Sci. 115, 335 (2008), Application(s): WB using rat cell lysates, Abstract
- Statins attenuate ischemia-reperfusion injury by inducing hemeoxygenase-1 in infiltrating macrophages: F. Gueler, et al.; Am. J. Pathol. 170, 1192 (2007), Application(s): IHC using rat tissue, Abstract
- Thiol antioxidants inhibit the adjuvant effects of aerosolized diesel exhaust particles in a murine model for ovalbumin sensitization: A.E. Nel, et al.; J. Immunol. 168, 2560 (2002), Application(s): WB using mouse samples, Abstract
- Impact of hyperthermic preconditioning on postischemic hepatic microcirculatory disturbances in an isolated perfusion model of the rat liver: K. Messmer, et al.; Hepatology 31, 407 (2000), Application(s): WB using rat samples, Abstract
- Overexpression of heme oxygenase-1 is neuroprotective in a model of permanent middle cerebral artery occlusion in transgenic mice: M.D. Maines, et al.; J. Neurochem. 72, 1187 (1999), Application(s): IHC using mouse samples, Abstract
- Neuronal overexpression of heme oxygenase-1 correlates with an attenuated exploratory behavior and causes an increase in neuronal NADPH diaphorase staining: K.J. McCoubrey, et al.; J. Neurochem. 70, 2057 (1998), Application(s): WB using mouse samples, Abstract
Related Products

Application | ELISA, IHC, WB |
---|---|
Host | Goat |
Species Reactivity | Mouse |
Datasheet, Manuals, SDS & CofA
Manuals And Inserts
Certificate of Analysis
Please enter the lot number as featured on the product label
SDS
Enzo Life Science provides GHS Compliant SDS
If your language is not available please fill out the SDS request form