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HIGHDEF^® red IHC chromogen (AP)

ADI-950-140

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High definition IHC chromogen producing brilliant red color for AP-activated development.

HIGHDEF^® red IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridization systems.

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HIGHDEF® red IHC chromogen (AP) Immunohistochemistry — Immunohistochemical analysis: Formalin fixed paraffin embedded human tonsil double stained using MULTIVIEW^® (mouse-HRP/rabbit-AP) IHC kit (Prod. no. ADI-950-100). Primary rabbit anti-Ki67 antibody was visualized with HIGHDEF^® red IHC chromogen (AP) (Prod. no. ADI-950-140), while primary mouse anti-HMW CK antibody was visualized with HIGHDEF^® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170, sold separately), producing a distinct golden yellow color contrasting with the bright red.

Product Details

Application	IHC, ISH (in situ hybridization)
Contents	HIGHDEF^® red IHC chromogen (AP) substrate buffer and chromogen, Empty mixing bottle
Technical Info / Product Notes	The HIGHDEF^® red IHC chromogen (AP) is a substrate-chromogen system designed to be used for either IHC or ISH when using alkaline phosphatase detection. The system produces a brilliant dark red color and is insoluble in organic solvents; therefore, sections can be dehydrated in alcohol, cleared in xylene (or a xylene-substitute), and permanently mounted. This chromogen substrate system may be used for both automation and manual use.

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Spatiotemporal expression of fibroblast growth factor 4 and 10 during the morphogenesis of deciduous molars in miniature pigs: Y. Wang, et al.; Arch. Oral Biol. 155, 105795 (2023), Abstract
Baseline circulating unswitched memory B cells and B-cell related soluble factors are associated with overall survival in patients with clear cell renal cell carcinoma treated with nivolumab within the NIVOREN GETUG-AFU 26 study: Carril-Ajuria, L., Desnoyer, A., et al.; J. Immunother. Cancer 10, (2022), Abstract
Tertiary lymphoid structures generate and propagate anti-tumor antibody-producing plasma cells in renal cell cancer: Meylan, M., Petitprez, F., et al.; Immunity 55, 527 (2022), Abstract
Exploring human splenic red pulp vasculature in virtual reality: details of sheathed capillaries and the open capillary network: B.S. Steiniger, et al.; Histochem. Cell Biol. 155, 341 (2021), Abstract — Full Text
Association of AXL and PD-L1 Expression with Clinical Outcomes in Patients with Advanced Renal Cell Carcinoma Treated with PD-1 Blockade: Terry, S., Dalban, C., et al.; Clin. Cancer Res. 27, 6749 (2021), Abstract
Transforming Growth Factor-β Signaling Regulates Tooth Root Dentinogenesis by Cooperation With Wnt Signaling: Zhang, R., Lin, J., et al.; Front Cell Dev Biol 9, 687099 (2021), Abstract
B cells are associated with survival and immunotherapy response in sarcoma: F. Petitprez, et al.; Nature 577, 556 (2020), Abstract
Cannabinoid receptor 1 knockout alleviates hepatic steatosis by downregulating perilipin 2: K. Irungbam, et al.; Lab. Invest. 100, 454 (2020), Abstract — Full Text
Cholestasis impairs hepatic lipid storage via AMPK and CREB signaling in hepatitis B virus surface protein transgenic mice: K. Irungbam, et al.; Lab. Invest. 100, 1411 (2020), Abstract — Full Text
Nuclear immunoreactivity of BLM-s, a proapoptotic BCL-2 family member, is specifically detected in salivary adenoid cystic carcinoma: M.S. Tsai, et al.; Hum. Pathol. 84, 81 (2019), Abstract

Last modified: May 29, 2024

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HIGHDEF^® red IHC chromogen (AP)

ADI-950-140

Product Details

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HIGHDEF® red IHC chromogen (AP)

ADI-950-140

Product Details

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