High definition IHC chromogen producing brilliant red color for AP-activated development.
HIGHDEF® red IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridization systems.
Shipping: Available products typically ship within 24/48h, via priority shipping.
Do you need support? Contact Customer Service or Technical Support.
Online Account
Access or Create Your Account

Immunohistochemical analysis: Formalin fixed paraffin embedded human tonsil double stained using MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit (Prod. no. ADI-950-100). Primary rabbit anti-Ki67 antibody was visualized with HIGHDEF® red IHC chromogen (AP) (Prod. no. ADI-950-140), while primary mouse anti-HMW CK antibody was visualized with HIGHDEF® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170, sold separately), producing a distinct golden yellow color contrasting with the bright red.

Product Details
Application |
IHC, ISH (in situ hybridization) |
---|---|
Contents |
HIGHDEF® red IHC chromogen (AP) substrate buffer and chromogen, Empty mixing bottle |
Technical Info / Product Notes |
The HIGHDEF® red IHC chromogen (AP) is a substrate-chromogen system designed to be used for either IHC or ISH when using alkaline phosphatase detection. The system produces a brilliant dark red color and is insoluble in organic solvents; therefore, sections can be dehydrated in alcohol, cleared in xylene (or a xylene-substitute), and permanently mounted. This chromogen substrate system may be used for both automation and manual use. |
Handling & Storage
Long Term Storage |
+4°C |
---|---|
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
---|
- Spatiotemporal expression of fibroblast growth factor 4 and 10 during the morphogenesis of deciduous molars in miniature pigs: Y. Wang, et al.; Arch. Oral Biol. 155, 105795 (2023), Abstract
- Baseline circulating unswitched memory B cells and B-cell related soluble factors are associated with overall survival in patients with clear cell renal cell carcinoma treated with nivolumab within the NIVOREN GETUG-AFU 26 study: Carril-Ajuria, L., Desnoyer, A., et al.; J. Immunother. Cancer 10, (2022), Abstract
- Tertiary lymphoid structures generate and propagate anti-tumor antibody-producing plasma cells in renal cell cancer: Meylan, M., Petitprez, F., et al.; Immunity 55, 527 (2022), Abstract
- Exploring human splenic red pulp vasculature in virtual reality: details of sheathed capillaries and the open capillary network: B.S. Steiniger, et al.; Histochem. Cell Biol. 155, 341 (2021), Abstract — Full Text
- Association of AXL and PD-L1 Expression with Clinical Outcomes in Patients with Advanced Renal Cell Carcinoma Treated with PD-1 Blockade: Terry, S., Dalban, C., et al.; Clin. Cancer Res. 27, 6749 (2021), Abstract
- Transforming Growth Factor-β Signaling Regulates Tooth Root Dentinogenesis by Cooperation With Wnt Signaling: Zhang, R., Lin, J., et al.; Front Cell Dev Biol 9, 687099 (2021), Abstract
- B cells are associated with survival and immunotherapy response in sarcoma: F. Petitprez, et al.; Nature 577, 556 (2020), Abstract
- Cannabinoid receptor 1 knockout alleviates hepatic steatosis by downregulating perilipin 2: K. Irungbam, et al.; Lab. Invest. 100, 454 (2020), Abstract — Full Text
- Cholestasis impairs hepatic lipid storage via AMPK and CREB signaling in hepatitis B virus surface protein transgenic mice: K. Irungbam, et al.; Lab. Invest. 100, 1411 (2020), Abstract — Full Text
- Nuclear immunoreactivity of BLM-s, a proapoptotic BCL-2 family member, is specifically detected in salivary adenoid cystic carcinoma: M.S. Tsai, et al.; Hum. Pathol. 84, 81 (2019), Abstract
- CD8+ T cells regulate tumour ferroptosis during cancer immunotherapy: W. Wang, et al.; Nature 569, 270 (2019), Abstract — Full Text
- Prevalence of polyreactive innate clones among graft-infiltrating B cells in human cardiac allograft vasculopathy: D. Chatterjee, et al.; J. Heart Lung Transplant. 37, 385 (2018), Application(s): Multiplex IHC staining on human cardiac tissue, Abstract
- Preparation and characterization of gellan gum/glucosamine/clioquinol film as oral cancer treatment patch: W. Tsai, et al.; Mater. Sci. Eng. C Mater. Biol. Appl. 82, 317 (2018), Abstract
- Downregulation of renal tubular Wnt/β-catenin signaling by Dickkopf-3 induces tubular cell death in proteinuric nephropathy: D.W. Wong, et al.; Cell Death Dis. 7, e2155 (2016), Application(s): Immunohistochemical staining, Abstract
- Molecular signals involved in human B cell migration into the retina: in vitro investigation of ICAM-1, VCAM-1, and CXCL13: A.S. Bharadwaj, et al.; Ocul. Immunol. Inflamm. 5, 1 (2016), Abstract
Related Products
HIGHDEF® red IHC chromogen (AP, plus)
ADI-950-141
High definition IHC chromogen producing brilliant red color for AP-activated development in automated and manual systems.

HIGHDEF® blue IHC chromogen (AP)
ADI-950-150
High definition IHC chromogen producing brilliant blue color for AP-activated development.

HIGHDEF® blue IHC chromogen (HRP)
ADI-950-151
Unique high definition blue IHC chromogen for HRP-activated development.

HIGHDEF® yellow IHC chromogen (HRP)
ADI-950-170
Unique high definition yellow IHC chromogen for HRP-activated development.

Last modified: May 29, 2024
Datasheet, Manuals, SDS & CofA
Certificate of Analysis
Please enter the lot number as featured on the product label
SDS
Enzo Life Science provides GHS Compliant SDS
If your language is not available please fill out the SDS request form